去甲亚精胺对白色念珠菌生物膜形成的抑制作用

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Abstract Objective: To study the effect of norspermidine on Candida albicans (C.albicans) biofilm formation and the underlying mechanisms. Methods: Crystal violet staining, XTT assay, colony-forming unit (CFU) count, scanning electron microscope (SEM) imaging and qRT-PCR was conducted to detect the effect of norspermidine on biofilm biomass, metabolic activity, colony number, fungal morphology and virulence-related gene expression of C.albicans biofilm. Results: Crystal violet staining results demonstrated that different concentrations of norspermidine had different inhibitory effects on C.albicans biofilm formation. XTT assay showed that norspermidine significantly restrained the metabolic activity of C.albicans biofilm. CFU counting results indicated that norspermidine severely reduced the number of C.albicans colonies in biofilm. SEM imaging displayed that norspermidine dramatically disturbed the formation of C.albicans biofilm and norspermidine in high concentration promoted the occurrence of yeast phase; qRT-PCR showed that norspermidine substantially interfered with the gene expression of ywp, hwp and als3, which were related to the virulence of C.albicans. Conclusion: Norspermidine could be used as a potential polyamine drug for anti- C.albicans biofilm as it could not only inhibit the formation of C.albicans biofilm but also interfere with the expression of its virulence-associated gene expression.

Key words： norspermidine candida albicans biofilm gene expression

Received: 10 February 2019

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	CAO Yanfan
	ZHANG Keke
	ZHANG Yanni
	TANG Fengyu
	HU Fengting
	YE Qingsong.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2019/V49/I5/321

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