沉默信息调节因子3在丹酚酸A抗紫外线诱导视网膜色素上皮细胞氧化应激性损伤中的作用及机制

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Abstract Objective: To investigate the effect and mechanism of salvianolic acid A (Sal A) on retinal pigment epithelial cells (RPE) injured by ultra-violet (UV) radiation via upregulating SIRT3 expression. Methods: ARPE-19 cells were divided into five groups: control group, UV group, Sal A+UV group, Sal A+negative control si-RNA+UV group, Sal A+si-SIRT3+UV group. The dose of UV irradiation was 30 mJ/cm2 and the concentration of Sal A was 50 μmol/L. The cell viability was tested by MTT. The ROS content was detected by DCFH-DA straining. The activity of SOD2 was analyzed by CuZn/Mn-SOD activity detection kit (WST-8 method). The expression of SIRT3, SOD2, cleavage-Caspase 3 and cytochrome c (Cyt c) was analyzed by Western blot. The expression of SIRT3 and SOD2 mRNA was detected by RT-qPCR. Results: The expression of SIRT3 protein and mRNA in ARPE-19 cells was decreased by UV irradiation. Pretreatment with 5, 25 and 50 μmol/L reversed the expression of SIRT3 protein and mRNA by UV. Compared with the control group, the apoptosis rate, ROS content, the expression of Bax, cleaved-caspase 3 and Cyt c in UV group were significantly increased, while the cell survival rate, the expression of SOD2 and Bcl-2, the activity of SOD2 were significantly decreased in UV group. Compared with UV group, the apoptosis rate, ROS content, the expression of Bax, cleaved-Caspase 3 and Cyt c were significantly decreased, while the cell survival rate, the expression of SOD2 and Bcl-2, the activity of SOD2 were significantly increased in Sal A+UV group. Compared with Sal A+UV group, the expression of Bax, cleaved-Caspase 3 and Cyt c in UV group were significantly increased, while the cell survival rate, the expression of SOD2 and Bcl-2, the activity of SOD2 were significantly decreased in Sal A+siSIRT3+UV group. Conclusion: Sal A inhibited cell damage induced by UV via up-regulating SIRT3 expression and reversing SOD2 activity.

Key words： salvianolic acid A silent information regulator 3 superoxide dismutase 2 cell injury retinal pigment epithelium cells

Received: 10 October 2018

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	LI Xia
	WANG Yanfang
	CHEN Zhanqiao
	YU Songping.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2019/V49/I4/272

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