结肠癌不同转移潜能细胞株SW620及SW480的代谢组学研究

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Abstract Objective: To study the metabolic profiles of human colorectal carcinoma cell lines with different metastatic potentials and analyze the relationship between metabolites and the metastasis of colorectal carcinomas. Methods: The characteristic alterations in metabolites of two isogenic colorectal cancer cell lines (highly metastatic SW620 and nonmetastatic SW480) were explored by the PLS-DA model based on the 1H NMR spectra. Moreover, Seahorse XF analyzer was used to assess ECAR and OCR. Results: The metabolic profile of SW620 and SW480 cell lines displayed obvious differences along in the PC1 direction. Compared with SW480, lactate, succinic, isoleucine, acetate, glycine and glutamine were increased, and phosphorylcholine, ATP, inositol and taurine were reduced in SW620 cells (P<0.05). SW620 cells with high metastatic potential have higher levels of glycolysis, TCA cycle activity, ECAR and OCR levels. Conclusion: The metabolic profile of SW620 cells with high metastatic potential shows clear differences from SW480 cells, and a variety of metabolites can be involved in the metastasis of colorectal carcinoma. Compared with SW480 cells, SW620 has a higher metabolic base potential, suggesting that it needs energy for high metastatic ability. Meanwhile, sw620 can adapt to its microenvironment more quickly, suggesting that the occurrence of metastasis is closely related to metabolism.

Key words： colorectal carcinoma SW620 SW480 1H nuclear magnetic resonance metabonomics

Received: 08 October 2018

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	NIU Yan
	ZHOU Yi
	ZHENG Hong
	ZHAO Liangcai
	LI Chen
	GAO Hongchang.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2019/V49/I4/243

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