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| HLA-G expression and its clinical significance by HBV |
Department of ICU, Luqiao Hospital of Enze Medical Center, Taizhou, 318050
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ZHOU Linjie,ZHENG Zhencang,WANG Xin, et al. HLA-G expression and its clinical significance by HBV[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(6): 435-.
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Abstract Objective: To investigate HLA-G expression and its clinical significance by HBV. Methods: Real-time RT-PCR and enzyme-linked immunosorbent assay (ELISA) were used to explore the serum HBV DNA load and plasma sHLA-G concentrations. HBV and THP-1 were cocultured for 1~6 d. RT-PCR and Western blot were used to analyse the expression of HLA-G in THP-1. Results: Plasma sHLA-G levels were dramatically increased in the peripheral blood of patients with HBV when compared with that in normal controls (P<0.01). After in vitro infection by a highly pathogenic strain of HBV, HLA-G mRNA was dramatically increased and the protein expression of HLA-G was induced. Datas showed that HLA-G transcription and protein expression were induced in 1 day and reached highest on 4 day and then decreased in a manner of time-dependent. Conclusion: Given its immunotolerant property, an increase in HLA-G of patients with HBV may play an important role in regulation of the inflammatory response process in vivo.
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Received: 19 April 2013
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RUAN Yufei, LIU Qi, WU Bo, XU Zhiyong, CHEN Ke, JIANG Minzhi, ZHANG Junling,YU Jinsheng, CHEN Yiping.. Establishment and identification of acute hepatitis animal model infected by murine cytomegalovirus in C57BL/6 mice[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(5): 345-351,357. |
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