miR-202通过降低肝癌细胞ROCK1表达抑制其迁移和侵袭

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (1546 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To observe the effect of microRNA-202 on the migration and invasion of hepatocellular carcinoma (HCC) cells and explore the possible molecular mechanism. Methods: The expression level of miR-202 in human HCC cells (Hep3B, Huh7, HCCLM3) and human normal hepatic cells (LO2) was detected by quantitative real-time PCR (qRT-PCR). miR-202 mimic or control microRNA (miR-control) was transfected into the HCCLM3 cells and miR-202 inhibitor or negative control microRNA (miR-NC) was transfected into the Hep3B cells. Transfection efficiency was examined by qRT-PCR. The effect of miR-202 on the migration and invasion of HCC cells was detected by Transwell assay. Bioinformatics website TargetScan was used to predict the potential targets of miR-202 and dual luciferase reporter assay was used to validate the target gene of miR-202. qRT-PCR and Western blot were used to detect the expression of miR-202 target gene. Results: The expression of miR-202 in normal liver cells was significantly higher than that in HCC cells. Transfection of miR-202 mimics significantly increased the expression level of miR-202 in HCCLM3 cells, and led to the decreased ability of cell migration and invasion. Transfection of miR-202 inhibitor significantly decreased the expression level of miR-202 in Hep3B cells, and led to the increased ability of cell migration and invasion. Bioinformatics website and dual luciferase reporter gene assay confirmed ROCK1 was the target gene of miR-202. Overexpression of miR-202 significantly reduced the expression of ROCK1 while miR-202 knockdown led to increased expression of ROCK1. Conclusion: The expression of miR-202 is down-regulated in HCC cells. miR-202 inhibits the migration and invasion of HCC cells by down-regulating the expression of ROCK1 gene.

Key words： MicroRNA-202 liver cancer ROCK1 migration invasion

Received: 24 September 2018

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	YUAN Linlin
	YU Xiaofen.

URL:

https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2019/V49/I3/204

[1] 肖开银. 原发性肝癌流行病学研究进展[J]. 中国普外基础与临床杂志, 2000(4): 272-274.
[2] REIG M, DA FONSECA L G, FAIVRE S. New trials and results in systemic treatment of HCC[J]. J Hepatol, 2018, 69(2): 525-533.
[3] 应江辉, 蒋佩佩, 金灿灿, 等. microRNA-212对胃癌细胞系SGC7901迁移和侵袭能力的影响[J]. 温州医科大学学报, 2016, 46(9): 644-648.
[4] YATES L A, NORBURY C J, GILBERT R J. The long and short of microRNA[J]. Cell, 2013, 153(3): 516-519.
[5] BRACONI C, HENRY J C, KOGURE T, et al. The role of microRNAs in human liver cancers[J]. Semin Oncol, 2011, 38(6): 752-763.
[6] ZHAO Y, LI C, WANG M, et al. Decrease of miR-202-3p expression, a novel tumor suppressor, in gastric cancer[J]. PLoS One, 2013, 8(7): e69756.
[7] SUN Z, ZHANG T, HONG H, et al. miR-202 suppresses proliferation and induces apoptosis of osteosarcoma cells by downregulating Gli2[J]. Mol Cell Biochem, 2014, 397(1-2): 277-283.
[8] FARHANA L, DAWSON M I, FONTANA J A. Down regulation of miR-202 modulates Mxd1 and Sin3A repressor complexes to induce apoptosis of pancreatic cancer cells[J]. Cancer Biol Ther, 2015, 16(1): 115-124.
[9] ZHANG Y, ZHENG D, XIONG Y, et al. miR-202 suppresses cell proliferation in human hepatocellular carcinoma by downregulating LRP6 post-transcriptionally[J]. FEBS Lett, 2014, 588(10): 1913-1920.
[10] YANG N, EKANEM N R, SAKYI C A, et al. Hepatocellular carcinoma and microRNA: new perspectives on therapeutics and diagnostics[J]. Adv Drug Deliv Rev, 2015, 81: 62-74.
[11] FANG Y, XUE J L, SHEN Q, et al. MicroRNA-7 inhibits tumor growth and metastasis by targeting the phosphoinositide 3-kinase/Akt pathway in hepatocellular carcinoma[J]. Hepatology, 2012, 55(6): 1852-1862.
[12] YANG W, DOU C, WANG Y, et al. MicroRNA-92a contributes to tumor growth of human hepatocellular carcinoma by targeting FBXW7[J]. Oncol Rep, 2015, 34(5): 2576-2584.
[13] BANDIERA S, PFEFFER S, BAUMERT T F, et al. miR-122-a key factor and therapeutic target in liver disease[J]. J Hepatol, 2015, 62(2): 448-457.
[14] LOCHHEAD P A, WICKMAN G, MEZNA M, et al. Activating ROCK1 somatic mutations in human cancer[J]. Oncogene, 2010, 29(17): 2591-2598.
[15] ZHOU X, WEI M, WANG W. MicroRNA-340 suppresses osteosarcoma tumor growth and metastasis by directly targeting ROCK1[J]. Biochem Biophys Res Commun, 2013, 437(4): 653-8.
[16] MAJID S, DAR A A, SAINI S, et al. MicroRNA-1280 inhibits invasion and metastasis by targeting ROCK1 in bladder cancer[J]. PLoS One, 2012, 7(10): e46743.