新型姜黄素纳米胶束丝素凝胶的制备及其质量表征

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Abstract Objective: To prepare the curcumin-loaded nanomicelles with a novel polymer, RRR-a-tocopheryl succinate-grafted-ε-polylysine conjugate (VES-g-ε-PLL), and to characterize it to use silk fibroin as a hydrogel-based matrix to further facilitate topical delivery of curcumin in exploration of a promising potential of this drug delivery system in the treatment of psoriasis. Methods: VES-g-PLL nanomicelles encapsulated with curcumin were prepared by injection method, and the micromorphology, particle size, Zeta potential, encapsulation efficiency, drug loading, and release rate were evaluated. CUR-NMs-gel was then prepared by mixing the CUR-NMs with the SF solution followed by a sonication-induced gelation method. The microscopic morphology, in vitro drug release and skin permeability of CUR-NMs-gel were determined by scanning electron microscopy, HPLC and confocal laser scanning microscopy. Results: The CUR-NMs was granulate-like particles with uniform dispersion. The nanomicelles exhibited an ultra-small hydrodynamic diameter (31.14±7.86) nm and a positive Zeta potential (16.70±1.45) mV. Moreover, curcumin could effectively be encapsulated in the polymeric nanoparticles with encapsulating efficiency of 82.21%±4.32%. The CUR-NMs-gel displayed a 3D network with flocculate of CUR-NMs adhering to its surface. The CUR release curves from the CUR-NMs-gel exhibited an obvious sustained release of DTX in physiological media with no evidence of burst effect, and only 30% of CUR was released from the CUR-NMs-gel at 72 h in pH 7.4 PBS, while 49% of CUR was released from the CUR-NMs. Vivo studies on skin penetration showed that the application of CUR-NMs-gel can significantly increase the transdermal permeability of the drug compared with the curcumin solution gel. Conclusion: The permeable nanomicelles-gel system could realize the sustained release and transdermal absorption of the soluble drug curcumin, which suggested a promising potential of this drug delivery system to treat such inflammatory skin disorders as psoriasis.

Key words： curcumin silk fibroin nanomicelles psoriasis

Received: 31 August 2018

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	ZHONG Songyang
	ZHENG Hongliang
	MAO Kaili
	WANG Siwei.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2019/V49/I3/184

[1] LIU Y, YANG G, ZHANG J, et al. Anti-TNF-alpha monoclonal antibody reverses psoriasis through dual inhibition of inflammation and angiogenesis[J]. Int Immunopharmacol, 2015, 28(1): 731-743.
[2] HUSSAIN A R, AHMED M, AL-JOMA H A, et al. Curcumin suppresses constitutive activation of nuclear factor-kappa B and requires functional Bax to induce apoptosis in Burkitt's lymphoma cell lines[J]. Mol Cancer Ther, 2008, 7(10): 3318-3329.
[3] LAUTERBAC A, MULLER-GOYMANN C C. Applications and limitations of lipid nanoparticles in dermal and transdermal drug delivery via the follicular route[J]. Eur J Pharm Biopharm, 2015, 97: 152-163.
[4] ZHAO Y, BROWN M B, JONES S A. Pharmaceutical foams: are they the answer to the dilemma of topical nanoparticles?[J]. Nanomedicine, 2010, 6(2): 227-236.
[5] RAJA M A, ZEENAT S, ARIF M, et al. Self-assembled nanoparticles based on amphiphilic chitosan derivative and arginine for oral curcumin delivery[J]. Int J Nanomedicine, 2016, 11: 4397-4412.
[6] XU H L, MAO K L, LU C T, et al. An injectable acellular matrix scaffold with absorbable permeable nanoparticles improves the therapeutic effects of docetaxel on glioblastoma [J]. Biomaterials, 2016, 107: 44-60.
[7] ELZOGHBY A O. Gelatin-based nanoparticles as drug and gene delivery systems: reviewing three decades of research [J]. J Control Release, 2013, 172(3): 1075-1091.
[8] 孙颖. 姜黄素对TPA诱导的银屑病小鼠模型血清ASO和IL-8水平的影响研究[J]. 中国生化药物杂志, 2014, 7(34): 46-48.
[9] 范子梁, 金冰慧, 徐霞芳, 等. 包截姜黄素纳米胶束的制备与体外抗肿瘤评价[J]. 温州医科大学学报, 2017, 47(9): 625-630, 636.
[10] DAVIS M E, CHEN Z G, SHIN D M. Nanoparticle therapeutics: an emerging treatment modality for cancer[J]. Nat Rev Drug Discov, 2008, 7(9): 771-782.
[11] BEBER T C, ANDRADE D F, CHAVES P S, et al. Cationic polymeric nanocapsules as a strategy to target dexamethasone to viable epidermis: skin penetration and permeation studies[J]. J Nanosci Nanotechnol, 2016, 16(2): 1331-1338.
[12] BATHEJA P, SHEIHET L, KOHN J, et al. Topical drug delivery by a polymeric nanosphere gel: Formulation optimization and in vitro and in vivo skin distribution studies[J]. J Control Release, 2011, 149(2): 159-167.
[13] DONG Y, DONG P, HUANG D, et al. Fabrication and characterization of silk fibroin-coated liposomes for ocular drug delivery[J]. Eur J Pharm Biopharm, 2015, 91: 82-90.
[14] LU G, LIU S, LIN S, et al. Silk porous scaffolds with nanofibrous microstructures and tunable properties[J]. Colloids Surf B Biointerfaces, 2014, 120: 28-37.