红景天苷对诱导的成骨细胞在低氧环境中的保护作用

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Abstract Objective: To investigate the protective effect of salidroside on induced osteoblasts in hypoxia environment and its mechanism. Methods: the mouse fibroblasts were separated and cultured by tissue block method. The transfected induced pluripotent stem cells were reprogrammed and the QT-PCR of Nanog, Oct-4, Rexl and Sox-2 were detected to the induced osteoblasts differentiated from the induced pluripotent stem cells and identified by alizarin red staining. The induced osteoblasts were divided into three groups: salidroside preconditioning group, normal oxygen group and hypoxia group. Annexin V-FITC/PI staining was used to detect the apoptosis rate, and Western-blot was used to detect VEGF, HIF-1 alpha. Results: The expression of endogenous gene Nanog, Oct-4, Rexl and Sox-2 of induced pluripotent stem cells was higher than that of mouse fibroblasts cells. The result is Nanog (783±42 vs. 308±23), Oct-4 (774±37 vs. 319±31), Rexl (742±28 vs. 382±31), Sox-2 (831±31 vs. 394±12). Alizarin red staining showed that the induced osteoblasts cells had positive staining results. The apoptotic rate of salidroside group was significantly lower than that of hypoxia group [(35.65±3.92)% vs. (95.20±1.03)%]. There was significant difference between the two groups (P<0.05). The expression of HIF-1a and VEGF in salidroside group was higher than that in hypoxic group (0.93±0.05 vs. 0.81±0.02, 0.95±0.03 vs. 0.79±0.04, P<0.05). Conclusion: Salidroside has protective effect on hypoxic environment of osteoblasts.

Key words： salidroside induced pluripotent stem cells hypoxia osteoblast cells apoptosis

Received: 11 June 2018

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	SHEN Xinsheng
	CHEN Ou
	LAI Xianliang
	SU Jia

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2019/V49/I3/179

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