β-蜕皮甾酮对激素性骨质疏松大鼠血清骨代谢指标及骨密度的影响

doi:10.3969/j.issn.2095-9400.2019.02.005

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Abstract Objective: To observe the effects of β-Ecdysterone (β-Ecd) on serum biochemical indexes and bone mineral density (BMD) in rats with glucocorticoid-induced osteoporosis. Methods: A total of 30 male Wistar rats were randomly divided into five groups: control group, prednisolone (Pred) group, Pred+β-Ecd (5 mg/kg) group, Pred+β-Ecd (10 mg/kg) group and Pred+alendronate (ALN) group (3 mg/kg) with 6 rats in each group. The concentration of calcium and phosphorus, the activities of antitartrate phosphatase (TRAP) and alkaline phosphatase (ALP) in serum of rats in each group were determined by automatic biochemistry analyzer. An analysis of bone structure and BMD of the fifth lumber vertebra of rats in each group was made by Micro-CT. Results: Compared with the control group, the serum calcium and phosphorus levels in Pred group significantly increased and the serum ALP activity and TRAP activity increased. Compared with Pred group, Pred+ALN group and Pred+β-Ecd (10 mg/kg) group had lower serum calcium and phosphorus levels, lower serum ALP activity and lower TRAP activity. In Pred+β-Ecd (5 mg/kg) group, the activity of ALP and the level of TRAP decreased, but the levels of calcium and phosphorus were not significantly affected. Bone structure and BMD Micro-CT results showed that different degree of bone loss was observed in all Pred treated rats. In the Pred group, the BMD value of the fifth lumbar vertebra was significantly lower than that of the control group. Micro-CT evaluation showed that the ratio of bone surface area to tissue volume (BS/TV) in Pred group was lower than that in control group. Three-dimensional model analysis showed that the number of trabecular bone (Tb.N) and bone volume fraction (BV/TV) in prednisolone group were significantly lower than those in control group. The trabecular separation degree (Tb.Sp) was significantly increased in prednisolone group. However, there was no significant difference in bone trabecular thickness (Tb.Th) and structural model index (SMI). Compared with Pred group, BMD, BS/TV, BV/TV and Tb.N levels in Pred+ALN group and Pred+β-Ecd (10 mg/kg) group significantly increased, but Tb.Sp and SMI levels decreased. Compared with Pred group, the levels of BMD, BS/TV, BV/TV and Tb.N in Pred+β-Ecd (5 mg/kg) group were significantly higher, but Tb.Th and Tb.Sp levels were lower. Conclusion: β-Ecd may be applied to the treatment of glucocorticoid osteoporosis by prohibiting bone loss and improving bone metabolism.

Key words： β-Ecdysone glucocorticoid osteoporosis bone mineral density rats bone metabolism

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	TANG Yanghua
	XIN Dawei
	LI Guosong
	YUE Zhenshuang
	ZENG Linru

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2019.02.005 OR https://xb.wmu.edu.cn/EN/Y2019/V49/I2/100

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