膜微粒抑制缺氧无血清诱导的骨髓间充质干细胞的凋亡

doi:10.3969/j.issn.2095-9400.2019.02.001

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Abstract Objective: To investigate the effects of myocardial infarction plasma-derived microparticles (MPs) on the hypoxia and serum deprivation induced apoptosis of bone marrow mesenchymal stem cells (BMSCs). Methods: Myocardial infarction (MI) was created by left anterior descending artery ligation in Lewis rats. The plasma-derived MPs were isolated 24 hours later from the blood. In order to study the protective effects of MPs on BMSCs under the conditions of hypoxia and serum deprivation (hypoxia/SD). BMSCs were pretreated with different concentrations (0.5 μg/mL, 1 μg/mL, and 2 μg/mL) of MPs before the apoptosis was induced. Cell apoptosis was evaluated by using flow cytometry of Annexin V/PI staining, Hoechst staining and the Tunel assay. Expression of cleavage of caspase-3 were assessed by Western blotting. The Akt pathway inhibitor AZD5363 was used to reveal the mechanism of MPs inhibiting BMSCs apoptosis. Results: The MPs derived from the myocardial infarction rats could remarkably prevent BMSCs from Hypoxia/SD induced apoptosis through activating Akt signaling pathway, and its anti-apoptosis effect showed a concentration dependence (0.5-2 μg/mL) (P<0.01). Conclusion: MPs derived from myocardial infarction could effectively inhibit hypoxia/SD induced apoptosis of BMSCs.

Key words： mesenchymal stem cells myocardial infarction microparticles apoptosis rats

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	JIN Peifeng1
	JIANG Sheng
	WENG Jiakan
	WANG Lei
	Ding Lu
	ZHAO Kaixiang
	SUN Chengchao

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2019.02.001 OR https://xb.wmu.edu.cn/EN/Y2019/V49/I2/79

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