汉黄芩素对急性肺损伤小鼠的保护作用

doi:10.3969/j.issn.2095-9400.2019.01.009

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Abstract Objective: To explore the therapeutic effect of wogonin on mice with LPS-induced acute lung injury and its possible mechanism. Methods: The acute lung injury model was constructed by LPS (1 μg/kg) endotracheal instillation using C57/BL6 mice. The experiment was composed of 3 groups: control group, LPS-induced acute lung injury group (LPS group), and wogonin treatment group (WOG group). Flow cytometry and ELISA were used to detect the content of immune cells (neutrophils and macrophages) and the secretion of inflammatory cytokines in bronchoalveolar lavage fluid (BALF). Lung tissues of mice in each experimental group were also collected for hematoxylin-eosin (HE) staining. Finally, the molecular mechanism of the protective effect of wogonin on acute lung injury mice was investigated by analyzing the common downstream pathway of LPS, namely NF-κB signaling pathway. Results: Wogonin can significantly reduce the wet-to-dry (W/D) ratio of lung tissue (P<0.05), the infiltration of inflammatory cells in lung tissue, and the production of TNF-α, IL-1β, IL-6 and IL-10 inflammatory cytokine (P<0.01). The results of Western blot showed that wogonin can significantly inhibit the NF-κB signaling pathway. Conclusion: Wogonin possesses potential protection against LPS-induced acute lung injury through its anti-inflammatory property, and its potential mechanism is associated with NF-κB signaling pathways.

Key words： wogonin lipopolysaccharide acute lung injury inflammatory response NF-κB mice

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	GE Jinlin
	YU Wenwen
	ZENG Yufeng
	JINChenci
	DAI Yuanrong

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https://xb.wmu.edu.cn/EN/10.3969/j.issn.2095-9400.2019.01.009 OR https://xb.wmu.edu.cn/EN/Y2019/V49/I1/38

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