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The mechanism of Butein in promoting apoptosis and inhibiting metastasis of osteosarcoma cells |
LIN Zeng, PAN Tianlong, WU Dengying, KANG Xiaodiao, CAI Ningyu, PAN Jun. |
Department of Orthopaedics, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, 325027 |
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Cite this article: |
LIN Zeng,PAN Tianlong,WU Dengying, et al. The mechanism of Butein in promoting apoptosis and inhibiting metastasis of osteosarcoma cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(12): 917-920.
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Abstract Objective: To study the effects of Butein on apoptosis and invasion and metastasis of U2OS osteosarcoma cells. Methods: U2OS osteosarcoma cells were treated with different doses of Butein for 24 h respectively. CCK-8 assay was used to detect the activity of U2OS cells. Western blot assay was used to detect the expression of Bcl-2, Bax, MMP-2, MMP-9, and the PI3K-Akt signaling pathway related proteins. The TUNEL assay was used to detect the apoptosis of U2OS osteosarcoma cells. The above experiments were repeated three times to ensure the accuracy of the experimental results. Results: After U2OS osteosarcoma cells were treated with Butein, the cell activity of U2OS osteosarcoma was decreased significantly. The expression of Bax proteinwas increased, while the expression of Bcl-2, MMP-2 and MMP-9 was decreased. In addition, when U2OS osteosarcoma cells were treated with Butein, the expression of PI3K-Akt signaling pathway was decreased. Conclusion: Butein promotes the apoptosis of U2OS osteosarcoma and suppresses its invasion and metastasis. The results may suggest that Butein exhibits antitumor activities by suppressing the PI3K-Akt signaling pathway.
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Received: 11 June 2018
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