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The role of IGFBP-4 in breviscapine inhibited lung cancer A549 cells growth |
SUN Lei, LI Weiwen, LI Yuan, LAN Xiu, LYU Zhuqing |
Department of Respiratory Medicine, Lishui Hospital of Zhejiang University, the Fifth Affiliated Hospital of Wenzhou Medical University, Lishui Municipal Central Hospital, Lishui, 323000 |
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Cite this article: |
SUN Lei,LI Weiwen,LI Yuan, et al. The role of IGFBP-4 in breviscapine inhibited lung cancer A549 cells growth[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(11): 813-817.
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Abstract Objective: To investigate the role of IGFBP-4 in breviscapine (BVP) inhibited lung cancer A549 cells growth. Methods: A549 cells were treated with BVP 0, 25, 50 and 100 μmol/L BVP for 48 h. Adv-IGFBP-4-OE™ DNA, EGFP and Adv-KD™ Easy RNAi EGFP were used to establish IGFBP-4 over- or down- expression A549 cells. MTT assay was used to detect cell survival rate, Trypan blue staining was applied to analyze cell death rate, Annexin V/Propidium iodide (PI) double staining was used to test cell apoptosis. Western blot, RT-qPCR and ELISA were used to detect the expression Bax, Bcl-2, the protein and mRNA expression and secretion of IGFBP-4. Results: The viability decreased, but apoptosis rate increased significantly in A549 cells with 25, 50 and 100 μmol/L BVP treatment. BVP also increased Bax but reduced Bcl-2 expression. The mRNA and protein expression of IGFBP-4 and the secretion of IGFBP-4 increased with BVP treatment for 48 h. The over-expression of IGFBP-4 decreased the survival rate of A549 cells, and enhanced the growth inhibition by 100 μmol/L BVP. The down-expression of IGFBP-4 increased the cell survival rate, and alleviated the growth inhibition by 100 μmol/L BVP. Conclusion: BVP has an inhibitory effect on A549 growth, which might be related to overexpression of IGFBP-4.
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Received: 06 May 2018
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[1] CHEN W, ZHANG S, ZOU X. Evaluation on the incidence, mortality and tendency of lung cancer in China[J]. Thorac Cancer, 2010, 1(1): 35-40.
[2] ZHENG R, ZENG H, ZUO T, et al. Lung cancer incidence and mortality in China, 2011[J]. Thoracic Cancer, 2016, 7 (1): 94-99.
[3] MOORE B S, CARTER G T, BRÖNSTRUP M. Editorial: Are natural products the solution to antimicrobial resis-tance?[J]. Nat Prod Rep, 2017, 34(7): 685-686.
[4] ABDELMOHSEN U R, BALASUBRAMANIAN S, OELSCHLAEGER T A, et al. Potential of marine natural products against drug-resistant fungal, viral, and parasitic infec-tions[J]. Lancet Infect Dis, 2017, 17(2): e30-e41.
[5] WU Y, FAN Q, LU N, et al. Breviscapine-induced apoptosis of human hepatocellular carcinoma cell line HepG2 was involved in its antitumor activity[J]. Phytother Res, 2010, 24 (8): 1188-1194.
[6] WANG Q, WANG Q, WANG S F, et al. Oral Chinese herbal medicine as maintenance treatment after chemotherapy for advanced non-small-cell lung cancer: a systematic review and meta-analysis[J]. Curr Oncol, 2017, 24(4): e269-e76.
[7] ZENG J, CAI S. Breviscapine suppresses the growth of non-small cell lung cancer by enhancing microRNA-7 expres-sion[J]. J Biosci, 2017, 42(1): 121-129.
[8] KE Y, BAO T, WU X, et al. Scutellarin suppresses migration and invasion of human hepatocellular carcinoma by inhibiting the STAT3/Girdin/Akt activity[J]. Biochem Biophys Res Commun, 2017, 483(1): 509-515.
[9] GUAN Y B, YANG D R, NONG S J, et al. Breviscapine (BVP) inhibits prostate cancer progression through damaging DNA by minichromosome maintenance protein-7 (MCM-7) modulation[J]. Biomed Pharmacother, 2017, 93: 103-116.
[10] DAMON S E, MADDISON L, WARE J L, et al. Overexpression of an inhibitory insulin-like growth factor binding protein (IGFBP), IGFBP-4, delays onset of prostate tumor formation[J]. Endocrinology, 1998, 139(8): 3456-3464.
[11] DURAI R, YANG S Y, SEIFALIAN A M, et al. Role of insulin-like growth factor binding protein-4 in prevention of colon cancer[J]. World J Surg Oncol, 2007, 5: 128.
[12] XIAO Y, ZHU S, YIN W, et al. IGFBP-4 expression is adversely associated with lung cancer prognosis[J]. Oncol Lett, 2017, 14(6): 6876-6880.
[13] SATO H, SAKAEDA M, ISHII J, et al. Insulin-like growth factor binding protein-4 gene silencing in lung adenocarci-nomas[J]. Pathol Int, 2011, 61(1): 19-27.
[14] LI W, SUN D, LV Z, et al. Insulin-like growth factor binding protein-4 inhibits cell growth, migration and invasion, and downregulates COX-2 expression in A549 lung cancer cells [J]. Cell Biol Int, 2017, 41(4): 384-391. |
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