妊娠糖尿病大鼠胎盘、子宫和心脏组织中Nrf2蛋白表达和氧化应激水平分析

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Abstract Objective: To analyze the varied expressions of Nrf2 and the level of oxidative stress in the placental, uterine and cardiac tissues from gestational diabetes mellitus (GDM) rats and gestational rats. Methods: Twenty pregnant SD rats were randomly divided into GDM group and normal group. GDM group rats were injected with streptozotocin intraperitoneally to induce GDM models, while rats in the control group were injected with the same amount of citric acid buffer intraperitoneally. All the rats were sacrificed at day 30 of pregnancy by anesthesia, and the placentae, uteruses and hearts were excised for protein extraction. Subsequently, the levels of insulin, SOD as well as GSH in different tissues were assayed by ELISA. The level of MDA was examined by the thiobarbital acid colorimetry. The expression level of Nrf2 was analyzed by Western blot. Results: The insulin and MDA from the placental, uterine tissues were higher in GDM group compared with the normal group (P<0.05), while GSH and SOD were lower (P<0.05). Also, the GDM rats exhibited higher Nrf2 protein level in the placental and uterine tissues compared with normal group (P<0.05). However, there was no significant difference in cardiac tissue between the two groups (P>0.05). Conclusion: GDM leads to insulin resistance, oxidative stress and the elevated Nrf2 expression in the placenta and uterine, which are unaffected in the hearts. Thus, it is speculated that GDM-induced oxidative stress and Nrf2 alteration differ among tissues.

Key words： diabetes, gestational oxidative stress Nrf2 insulin rats

Received: 10 November 2017

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	SUN Congcong
	ZHENG Jiayong
	WANG Wenhuan
	HU Yanjun
	ZHENG Jianqiong
	ZHANG Hongping

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I11/796

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