妊娠期大鼠镉暴露与妊娠高血压疾病的关系

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Abstract Objective: To explore the cadmium’s toxicological effect on development of pregnancy-related hypertensive disorders through cadmium exposure during pregnancy in rats. Methods: Pregnant SD rats were divided into three groups (0.25 mg/kg group, 0.5 mg/kg group and control group) given intraperitoneal injection of different dose of cadmium chilorde (0.25, 0.5 mg/kg) and saline every day from gestational day (GD) 4 to 19. Non-pregnant rats received an intraperitoneal injection of 0.5 mg/kg cadmium chilorde as non-pregnant controls. The effect of cadmium on systolic blood pressure was measured by BP-98A blood pressure meter. And the effect of cadmium on urinary albumin excretion was investigated by BCA protein assay kit. This study also investigated the morphological changes in placenta, kidneys, liver, brain by HE staining, and ultrastructure of placental trophoblast cells were observed with transmission electron microscopy in pregnant rat exposed to cadmium. Results: The systolic blood pressures of rats at 12 and 19 days of pregnancy was significantly elevated after injection of cadmium at dose of 0.5 mg/kg compared with that in control group and non-pregnant group at the same dose (P<0.05). The urinary albumin excretion of rats in 0.5 mg/kg group was significantly higher compared with that in other three groups at gestational day 19 (P<0.01). The morphological changes in placenta induced by cadmium included thickening of the media vessel walls, degeneration and excessive perivillous fibrin depositionin placental labyrinth. The swelling of the glomerular endothelia cells and thickening of the media renal vessel walls also were observed in kidney. Reduced placental trophoblast intracellular organelles and papilla were revealed by electron microscope. Conclusion: The key features of pregnancy-related hypertension can be observed in rat after injection with a relatively low concentration of cadmium. Through further evaluation of the reproductive toxicological characteristics of cadmium during pregnancy, it is found that cadmium induces pathological damage in the placenta, kidney, liver, and brain of rats, which is similar to pregnancy-related hypertension in human in pathophysiological aspects.

Key words： pregnancy-related hypertension cadmium placental trophoblast cells pathology rats

Received: 12 March 2018

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	Wang Fan
	Qiu Haifan
	Liu Yi
	Chen Lulu
	Fan Fengyun
	Wang Zongmin

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I11/781

[1] 詹珍洁, 陈建业, 管克. 温州市瓯海区市售蔬菜中铅、镉、砷检测结果分析[J]. 中国卫生检验杂志, 2017, 27(6): 866-868.
[2] PANDYA C, PILLAI P, NAMPOOTHIRI L P, et al. Effect of lead and cadmium co-exposure on testicular steroid metabolism and antioxidant system of adult male rats[J]. Andrologia, 2012, 44 Suppl 1: 813-822.
[3] SARWAR M S, AHMED S, ULLAH M S, et al. Comparative study of serum zinc, copper, manganese, and iron in preeclamptic pregnant women[J]. Biol Trace Elem Res, 2013, 154(1): 14-20.
[4] 汪纪仓, 朱华丽, 林霖, 等. 镉致大鼠血液、肝脏、肾脏和睾丸的毒性损伤[J]. 毒理学杂志, 2017, 31(1): 10-13.
[5] 楼哲丰, 金龙金, 陈锡文, 等. 镉对小鼠睾丸精子生成的影响及其分子机制[J]. 温州医学院学报, 2007, 37(5): 455-457.
[6] 季星岐, 韩邦兴, 陈乃富, 等. 镉导致高血压发生机制的研究进展[J]. 生物学杂志, 2017, 34(4): 94-97, 109.
[7] MIKOLIĆ A, PIASEK M, SULIMANEC G A, et al. Oral cadmium exposure during rat pregnancy: assessment of transplacental micronutrient transport and steroidogenesis at term[J]. J Appl Toxicol, 2015, 35(5): 508-519.
[8] RAGHUPATHY R. Cytokines as key players in the pathophysiology of preeclampsia[J]. Med Princ Pract, 2013, 22 Suppl 1: 8-19.
[9] WANG F, ZHANG Q, ZHANG X, et al. Preeclampsia induced by cadmium in rats is related to abnormal local glucocorticoid synthesis in placenta[J]. Reprod Biol Endocrinol, 2014, 12: 77.
[10] ZHANG X, XU Z, LIN F, et al. Increased oxidative DNA damage in placenta contributes to cadmium-induced preeclamptic conditions in rat[J]. Biol Trace Elem Res, 2016, 170(1): 119-127.
[11] ZHANG Q, HUANG Y, ZHANG K, et al. Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia[J]. Environ Pollut, 2016, 218: 770-782.
[12] SAKAMOTO M, YASUTAKE A, DOMINGO J L, et al. Relationships between trace element concentrations in chorionic tissue of placenta and umbilical cord tissue: potential use as indicators for prenatal exposure[J]. Environ Int, 2013, 60: 106-111.
[13] DÍAZ M C, GONZÁLEZ N V, ZANUZZI C N, et al. Lectinhistochemistry for detecting cadmium-induced changes in the glycosylation pattern of rat placenta[J]. Biotech Histochem, 2017, 92(1): 36-45.
[14] MIKOLIĆ A, PIASEK M, SULIMANEC G A, et al. Oral cadmium exposure during rat pregnancy: assessment of transplacental micronutrient transport and steroidogenesis at term[J]. J Appl Toxicol, 2015, 35(5): 508-519.
[15] ALVAREZ M M, CHAKRABORTY C. Cadmium inhibits motility factor-dependent migration of human trophoblast cells[J]. Toxicol In Vitro, 2011, 25(8): 1926-1933.
[16] KOSANOVIC M, JOKANOVIC M. The association of exposure to cadmium through cigarette smoke with pregnancy-induced hypertension in a selenium deficient population[J]. Environ Toxicol Pharmacol, 2007, 24(1): 72-78.
[17] KIPPLER M, TOFAIL F, GARDNER R, et al. Maternal cadmium exposure during pregnancy and size at birth: a prospective cohort study[J]. Environ Health Perspect, 2012, 120 (2): 284-289.
[18] HAOUEM S, EL H A. Effect of cadmium on lipid peroxidation and on some antioxidants in the liver, kidneys and testes of rats given diet containing cadmium-polluted radish bulbs [J]. J Toxicol Pathol, 2013, 26(4): 359-364.
[19] TRINCHELLA F, RIGGIO M, FILOSA S, et al. Cadmium distribution and metallothionein expression in lizard tissues following acute and chronic cadmium intoxication[J]. Comp Biochem Physiol C Toxicol Pharmacol, 2006, 144(3): 272-278.