乙酸钠通过抑制过度炎症反应保护乙醇诱导的小鼠胃黏膜损伤

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Abstract Objective: To observe the effect of acetate sodium on the gastric mucosal lesion induced by ethanol and investigate the underlying mechanism. Methods: Mice were randomly divided into normal control group, model group and acetate sodium group. The gastric mucosal inflammation model was made by intragastric administration of ethanol. The inflammatory cytokines IL-1β, TNFα and MCP-1 were detected by real time quantitative PCR (RT-qPCR). CCK-8 was detected to evaluate the effect of acetate on the alcohol-induced injury of GES-1 which is normal cell line of human gastric mucosa. We also observed the effect of acetate sodium on the LPS-induced inflammation in the mice macrophage cell line J774 by detecting the inflammatory cytokines, IL-1β, TNFα and MCP-1. Results: Compared with normal control group, the ethanol stimulation significantly induced gastric mucosal lesion. However, acetate sodium dramatically reduced this lesion. The RT-qPCR results showed that the increased expressions of IL-1β, TNFα and MCP-1 after ethanol administration were obviously attenuated by acetate sodium (P<0.01). CCK-8 results indicated that acetate was significantly protective against the lesion induced by alcohol. The results of in vitro inflammation experiment on J774 showed that acetate sodium reduced the up-regulated expressions of IL-1β, TNFα and MCP-1 (P<0.01). Conclusion: Acetate sodium can attenuate ethanol-induced in gastric mucosal lesion. The anti-inflammation and protection against acetate in gastric mucosa play important roles in the corresponding mechanism.

Key words： acetate sodium gastric mucosa inflammatory mediators ethanol mice

Received: 29 January 2018

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	SHI Yilu
	ZHANG Jiali
	QIAO Wanning
	CHEN Jie
	SU Wei
	ZHANG Zhengzheng
	DONG Renjie
	WANG Fangyan
	HUANG Yingpeng

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I10/759

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