|
|
|
| Clinical effect of early thawed frozen embryo in the 3rd day cultured in the 5th day |
| XI Haitao, CHEN Hua, ZHANG Fan, LIU Xiaoming, FU Yanghua, ZHAO Junzhao. |
| Department of Reproductive Center, the Second Affiliated Hospital & Yuying Chlidren’s Hospital of Wenzhou Medical University, Wenzhou, 325027 |
|
| Cite this article: |
|
XI Haitao,CHEN Hua,ZHANG Fan, et al. Clinical effect of early thawed frozen embryo in the 3rd day cultured in the 5th day[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(10): 727-730.
|
|
|
|
Abstract Objective: To examine the clinical transplantation outcomes of cleavage stage embryos thawed 2 days earlier for culture, in order to confirm the development potential of cryopreserved cleavage stage embryos. Methods: In this prospective clinical study, 122 cycles of ART treatment for infertility have been evaluated. Frozen embryos in blastocyst group thawed and then cultured in sequential media until blastocyst stage were compared with thawed embryos in cleavage stage group. Results: Clinical rates was significantly higher in blastocyst group (66.67%) compared with cleavage group (37.5%). No statistical differences were reported in implantation rates between the two groups. Conclusion: The results indicated that blastocyst formation after thawing of cleavage stage embryos is a good predictor for embryo viability and pregnancy outcome.
|
|
Received: 17 May 2018
|
| |
|
|
|
[1] WANG S S, SUN H X. Blastocyst transfer ameliorates live birth rate compared with cleavage-stage embryos transfer in fresh in vitro fertilization or intracytoplasmic sperm injection cycles: reviews and meta-analysis[J]. Yonsei Med J, 2014, 55(3): 815-25.
[2] BEHR B, POOL T, MILKI A, et al. Preliminary clinical experience with human blastocyst development in vitro without co-culture[J]. Hum Reprod, 1999, 14(2): 454-457.
[3] ALIKANI M, CALDERON G, TOMIKIN G, et al. Cleavage anomalies in early human embryos and survival after prolonged culture in-vitro[J]. Hum Reprod, 2000, 15(12): 2634-2643.
[4] BRAGA D P, SETTI A S, FIGUEIRA R C, et al. The importance of the cleavage stage morphology evaluation for blastocyst transfer in patients with good prognosis[J]. J Assist Reprod Genet, 2014, 31(8): 1105-1110.
[5] 吴燕虹, 赵军招. 冻融胚胎移植内膜准备的研究进展[J].温州医科大学学报, 2016, 46(2): 149-153.
[6] 陈华, 孙海翔, 王慧焱, 等. 自然周期与替代周期准备内膜对冷冻胚胎移植结局的影响[J]. 南京医科大学学报, 2010, 30(2): 245-248.
[7] 高辉, 孙正怡, 邓成艳, 等. 人工周期法与自然周期法准备内膜在复苏囊胚移植中效果的比较[J]. 生殖与避孕, 2008, 28(1): 54-57.
[8] AFLATOONIAN A, OSKOUIAN H, AHMADI S, et al. Can fresh embryo transfers be replaced by cryopreserved-thawed embryo transfers in assisted reproductive cycles? A randomized controlled trial[J]. J Assist Reprod Genet, 2010, 27(7): 357-363.
[9] RIENZI L, UBALDI F, IACOBELLI M, et al. Day 3 embryo transfer with combined evaluation at the pronuclear and cleavage stages compares favourably with day 5 blastocyst transfer[J]. Hum Reprod, 2002, 17(7): 1852-1855.
[10] PAPANIKOLAOU E G, D’HAESELEER E, VERHEYEN G, et al. Live birth rate is significantly higher after blastocyst transfer than after cleavage-stage embryo transfer when at least four embryos are available on day 3 of embryo culture?A randomized prospectivestudy[J]. Hum Reprod, 2005, 20(11): 3198-3203.
[11] SHEN C, SHU D, ZHAO X, et al. Comparison of clinical outcomes between fresh embryo transfers and frozen-thawed embryo transfers[J]. Iran J Reprod Med, 2014, 12(6): 409-414.
[12] 岳超敏, 方丛, 宫晓, 等. 囊胚解冻过夜培养后移植对妊娠结局的影响[J]. 中山大学学报(医学科学版), 2013, 34(3): 415-421 |
|
|
|
