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| Screening for novel peptides specifically binding to the surface of endometriosis cells |
| YAN Linzhi, CAO Dan, WANG Ledan, LI Wenju, LYU Jieqiang. |
| Department of Gynecology and Obstetrics, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027 |
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| Cite this article: |
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YAN Linzhi,CAO Dan,WANG Ledan, et al. Screening for novel peptides specifically binding to the surface of endometriosis cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(10): 708-711.
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Abstract Objective: A 7-mer phage display library was employed to isolate novel peptides that specifically bind to the surface of ectopic endometrium cells in vitro. Methods: Phage display technology with biopanning and rapid analysis of selective interactive ligands between ectopic and eutopic endometrium cells was utilized. After five rounds of biopanning, 50 phage clones were randomly selected and analyzed by enzyme-linked immunosorbent assay and DNA sequencing. A peptide-competitive inhibition assay was performed to identify the affinity of positive phages toward ectopic endometrium cells. Results: ①After ELISA, the OD450 value of 13 clones was >0.5, and these phase clones were sequenced. Six distinct sequences out of 13 clones were obtained and 5 clones had a consensus DNA sequence CGCACCCGCCTGCATACCCGC, with a corresponding amino acid sequence of RTRLHTR. ②The binding capacity of the polypeptide RTRLHTR for endometriosis cells was reduced in a peptide dose-dependent pattern. In contrast, the control peptide revealed no such specific binding activity. Conclusion: The polypeptide RTRLHTR screened by phage display technology may offer a new direction for early diagnosis and treatment of endometriosis.
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Received: 21 December 2017
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