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| In vitro culture identification and growth characteristics of rapidly aging SAMP6 mouse osteoblasts |
| WANG Jie1, WANG Xiaofeng2 |
| 1.Department of Radiology, Rui’an Hospital of Traditional Chinese Medicine, Wenzhou, 325200; 2.Department of Science and Education, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Wenzhou, 325000 |
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| Cite this article: |
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WANG Jie,WANG Xiaofeng.. In vitro culture identification and growth characteristics of rapidly aging SAMP6 mouse osteoblasts[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(10): 703-707.
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Abstract Objective: To study the mechanism of senile osteoporosis in SAMP6 mouse by in vitro isolation and culture of mouse osteoblasts derived from SAMP6 mouse. Methods: The skull of neonatal SAMP6 mice was isolated by mechanical separation. SAMP6 mouse osteoblasts were cultured by enzyme digestion. The morphology of SAMP6 mouse osteoblasts was observed by alkaline phosphatase (ALP) staining and Wright-Jimeza staining. SAMP6 mouse osteoblasts was identified by the staining of calcified nodules with safranin. Results: In this experiment, the growth curve of osteoblasts in SAPM6 mouse was shown as follows: 1~3 days was the latent adaptation period of osteoblasts in SAMP6 mouse, and it entered the logarithmic growth phase of cells in 3 to 7 days, and then became the plateau phase. The SAMP6 mouse osteoblast cultured in vitro was stained by R-J and observed by microscope. The cytoplasm showed blue particles, and the nucleus was stained with purple-brown, with an average of 1-3 nucleoli. ALP stained microscopy showed that the distribution of purple particles in the cytoplasm, alizarin red staining could be seen in the naked eye and the calcified orange nodules, indicating that isolation and culture of SAMP6 mouse cells were experimental osteoblasts. Conclusion: The osteoblasts of SAMP6 neonatal mouse isolated and cultured in vitro in this experiment were initially qualified and basically corresponded with the characteristics of osteoblasts, which has laid foundations for the mechanism study of SAMP6 mouse mimicking senile osteoporosis.
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Received: 08 April 2018
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