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Observation of the fluorescent signals in the organs of mice |
HUANG Zhaoshuai, LIN Wu, CHEN Xiaoming. |
Department of Pediatric Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015 |
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Cite this article: |
HUANG Zhaoshuai,LIN Wu,CHEN Xiaoming.. Observation of the fluorescent signals in the organs of mice[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(9): 680-683.
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Abstract Objective: To observe the fluorescent signals in the liver, spleen and lung of mice and the quenching effect of autofluorescence by copper sulfate. Methods: ①Cell experiments: RG2 (rat glioma cell line) cells were divided into the control and treatment groups. Following maturity and attachment, two groups were fixed by 4% PFA. The treatment group was treated with 1.0 mmol/L copper sulfate solution (ammonium acetate buffer) for 10 min while the control group was not treated by the solution mentioned above. After sealed with fluorescent gel, the fluorescent intensity of these two groups was observed. ②Animal experiments: C57/BL6 mice were divided randomly into normal group and tail vein injection group. In tail vein injection group, the mice were injected with 100 µL of RG2 cell suspension and were sacrificed on the fourth day. After cardiac perfusion, the lungs, livers, and spleens were collected for frozen sections. In normal group, after cardiac perfusion, the lungs, livers, and spleens of mice were collected directly. The fluorescent signals of each organ and the effect of copper sulfate solution were observed. Results: After the treatment of copper sulfate, the fluorescent signals of RG2 reduced. The fluorescence distribution in each organ in mice was different; there was almost no autofluorescence in the lung; no obvious autofluorescence in the liver; marked autofluorescence in the spleen, which could not be eliminated by the treatment of copper sulfate solution. In contrast, the green fluorescence signals in the lung and liver in the tail vein injection group could be observed obviously. Conclusion: Copper sulfate can reduce the fluorescent intensity of cells and remove the background signal of spleen to a certain extent; the lung and liver can serve as better organs for the observation of fluorescence signals.
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Received: 28 March 2018
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