醒脑静注射液对颅脑损伤大鼠认知功能的影响

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Abstract Objective: To investigate the effect of Xingnaojing injection on cognitive function of rats with traumatic brain injury and its related mechanisms. Methods: A total of 108 rats were randomly divided as sham-operation group, trauma group and treatment group, with 36 rats in each group. Rats were given an intraperitoneal injection of 1 mL normal saline in the sham-operation group and trauma group, while rats in treatment group were administered intraperitoneally with 5 mL/kg Xingnaojing injection once a day for 7 days. Escape latency was recorded using Morris water maze for 6 rats in each group. At 12, 24, 48, 72 and 168 h respectively after the model was established, 6 rats in each group were decapitated. Brain tissues surrounding traumatic lesion were obtained. Apoptotic neuronal cells were determined using TUNEL staining. Protein expressions of procaspase-3 were investigated using Western blot. Caspase-3 protein expressions were detected using immunohistochemical staining technique. Results: Percentage of apoptotic neuronal cells and protein expressions of procaspase-3 and caspase-3 in the rat brain tissues as well as escape latency of rats were significantly higher in the trauma group than in the sham-operation group (P<0.05). The preceding variables in rats were significantly lower in the treatment group than in the trauma group (P<0.05). Conclusion: Xingnaojing injection may improve cognitive function of rats with traumatic brain injury via inhibiting caspase-3 mediated neuronal cellular apoptosis.

Key words： Xingnaojing injection cognitive function neuronal cellular apoptosis rats

Received: 23 December 2017

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	CHEN Yihua
	CHENG Zhenyu
	SHAO Linhua
	HE Zhongping
	WANG Ruiquan
	FU Bin.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I9/652

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