内皮细胞特异性分子-1对内毒素致小鼠急性肺损伤时炎症反应的影响

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Abstract Objective: To investigate the effect of Endocan on lipopolysaccharide (LPS) induced acute lung injury (ALI). Methods: LPS was used to construct C57BL/6 mice ALI model by aerosol inhalation for 30 min, and Endocan was injected intraperitoneally at 30 min prior to LPS exposure. Mice in control group were treated with normal saline at an equal volume. The mice were sacrificed 24 h after aerosol inhalation. The ratio of lung wet weight to dry weight (W/D) was measured to assess the extent of pulmonary edema. Myeloperoxidase (MPO) activity in lung tissues was measured to determine Neutrophil infiltration. The level of intercellular adhesion molecule-1 (ICAM-1), tumor necrosis factor-α (TNF-α) and interleukins-6 (IL-6) in bronchoalveolar lavage fluid (BALF) were detected by ELISA. The ultrastructural changes were visualized by electron microscope. Results: Compared with the control group, the lung W/D, MPO activity and the concentrations of TNF-α, IL-6 and ICAM-1 were dramatically increased in LPS group [W/D: 5.62±0.95 vs. 4.08±0.76, MPO (U/g): 2.71±0.59 vs. 1.39±0.28, TNF-α (pg/mL): 140.36±10.01 vs. 79.65±6.23, IL-6 (pg/mL): 156.54±17.82 vs. 50.11±6.02, ICAM-1 (pg/mL): 107.05±13.92 vs. 57.31±8.02, P<0.01] which could be relieved by Endocan treatment [W/D: 4.38±0.47 vs. 5.62±0.95, MPO (U/g): 1.84±0.42 vs. 2.71±0.59, TNF-α (pg/mL): 113.07±8.49 vs. 140.36±10.01, IL-6 (pg/mL): 123.22±12.80 vs. 156.54±17.82, ICAM-1 (pg/mL): 89.44±10.92 vs. 107.05±13.92, P<0.01]. Electron microscope data showed obvious ultrastructure injury induced by LPS, which was greatly attenuated in Endocan group. Conclusion: Endocan can suppress inflammatory response in endotoxin-induced acute lung injury, which suggests that Endcan may be a potential protective agent for ALI.

Key words： Endocan lipopolysaccharides acute respiratory distress syndrome mice

Received: 07 November 2017

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	ZHANG Xiaolong
	WU Haiya
	CHEN Jie
	WU Chengyun.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I9/636

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