丙泊酚对TNF-α诱导的小胶质细胞HMGB1表达的调控作用

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Abstract Objective: To investigate the effect of propofol on the expression of HMGB1 of microglia BV-2 cells induced by TNF-α. Methods: MTT was used to detect cell proliferation of microglia treated by propofol in different concentrations (5, 10, 20, 50, 100, 150 and 200 μmol/L). Control group, TNF-α (20 ng/mL) group, and TNF-α (20 ng/mL) + propofol (10, 20 and 50 μmol/L) group were set up. The expression of HMGB1 and the phosphorylation of p38 in microglia were detected by RT-qPCR and Western blot after microglia was pretreated with propofol (10, 20 and 50 μmol/L) and then stimulated with TNF-α. Utilizing siRNA to silence p38 before it was treated with propofol and the expression of HMGB1 was examined by RT-qPCR and Western blot. Results:High concentration of propofol (150 and 200 μmol/L) inhibited the proliferation of microglial cells. Compared with the control group, the expression of HMGB1 was increased after TNF-α stimulation. The expression of
HMGB1 in TNF-α+propofol group was significantly reduced and presented dosage dependence compared with TNF-α group. Silencing p38 inhibited the TNF-α-induced expression of HMGB1 in microglia. There was no significant difference in HMGB1 expression between the group with silenced p38 alone and the propofol-treated group after silencing p38. Conclusion: Propofol can be used to regulate the expression of HMGB1 of microglial cells through activating p38 and thus affecting the advanced inflammatory response to brain injury.

Key words： propofol microglia BV-2 cells tumor necrosis factor alpha high mobility group protein B1

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	Articles by authors
	Zhang Mingxiao
	Huang Luping
	Jin Shenhui
	Chen Sijia
	Dai Qinxue.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I9/625

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