甲酸基肽受体2抑制滋养细胞增殖侵袭功能的机制

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Abstract Objective: To explore the preliminary mechanism and effect of FPR2 on the proliferation, migration and invasion of human extravillous trophoblastic cells (TEV-1). Methods: Overexpression of FPR2 RNA was constructed and transfected into human TEV-1 cells. MTT assay, transwell assay and scarification test were used to detect the cell proliferation, migration and invasion. RT-PCR and Western blot was used to detect the expression of NF-ĸB and MMP9 mRNA and protein. Results: Over-expression of FPR2 significantly increased the expression levels of FPR2 mRNA and protein in TEV-1 cells (P<0.001), indicating that the transfection was successful. Over-expression of FPR2 gene significantly inhibited the proliferation, invasion (P<0.01) and migration of TEV-1 cells (P<0.05). Over-expression of FPR2 gene significantly decreased the mRNA and protein levels of NF-ĸB and MMP9 in TEV-1 cells (P<0.001). Conclusion: These findings indicated that over-expression of FPR2 gene may perturb proliferation, migration and invasion through NF-ĸB/MMP9 pathway in TEV-1 cells.

Key words： lipoxin A4 formyl peptide receptor 2 extravillous trophoblast cell proliferation cell movement cell invasion

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	Articles by authors
	Zhao Shenzhi
	HUANG Xianping
	XIANG Huiqiu
	LIAO Tingting
	CHEN Jiajia
	XU Zhangye

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I8/552

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