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| Diagnostic role of Wnt pathway gene promoter methylation in non-small-cell lung cancer |
| CHENG Guangjia1, LIU Shunlin2, ZHU Guoliang3, WANG Jinzhi4. |
| 1.Department of Geriatrics, Fenghua People’s Hospital, Ningbo, 315500; 2.Department of Respiratory, the First Affiliated Hospital of Soochow University, Suzhou, 215600; 3.Department of Pathology, Huzhou First People’s Hospital, Huzhou, 313000; 4.Department of Cell Biology School of Medicine, Soochow University, Suzhou, 215007 |
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| Cite this article: |
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CHENG Guangjia,LIU Shunlin,ZHU Guoliang, et al. Diagnostic role of Wnt pathway gene promoter methylation in non-small-cell lung cancer[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(7): 499-503.
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Abstract Objective: The gene in the Wnt signaling pathway was analyzed to improve its role as a biomarker for the diagnosis of non-small-cell lung cancer (NSCLC). Methods: The methylation levels of SFRP1, SFRP2, WIF1 and PRKCB in 111 NSCLC patients were evaluated by quantitative methylation-specific PCR (qMSP), and combined with clinical data and TCGA database analysis. Results: Promoter methylation levels of four candidate genes were significantly higher in tumor tissues compared with the adjacent tissues. SFRP1, SFRP2 expression level in male patients was higher than female; the sensitivity and specificity were 83.3% and 96.0% combined with the methylation level of four in the diagnosis of NSCLC. Conclusion: A panel of Wnt signal pathway genes (SFRP1, SFRP2, WIF1 and PRKCB) had the potential as methylation biomarkers in the diagnosis of NSCLC.
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Received: 28 September 2017
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