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| The regulatory effect of survivin in amyloid precursor protein metabolism |
| JIANG Weiqing1, YUAN Xin1, LIU Chen2, REN Xiao1, WANG Yonggang1 |
| 1.Department of Neurology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127; 2.Department of Neurology, the First Affiliated Hospital of Zhengzhou University, Zhenzhou, 450052 |
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| Cite this article: |
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JIANG Weiqing,YUAN Xin,LIU Chen, et al. The regulatory effect of survivin in amyloid precursor protein metabolism[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(7): 495-498.
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Abstract Objective: To explore the regulatory effect of survivin in amyloid precursor protein (APP) metabolism. Methods: Immunofluorescence technique was conducted to observe whether there was colocalization of survivin and APP through confocal imaging. Cell transfection with APP or APP-survivin plasmids were conducted to detect: whehter survivin affected the expression of APP through western blot analysis, and whether survivin inhibited the cell apoptosis induced by overexpression of APP though flow cytometry. Results: Survivin colocalized with APP in cultured rat primary hippocampal neurons. Survivin inhibited the expression of APP in PC12 cells. Moreover, survivin inhibited the cell apoptosis induced by overexpression of APP. Conclusion: Survivin could inhibit the cellular protein expression of APP and could inhibit the cell apoptosis induced by overexpression of APP.
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Received: 24 January 2018
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