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| The different injection site determine the result of bone marrow mesenchymal stem cells transplantation for myocardial infarction |
| JIN Peifeng1, Wang Yehuan2, JIANG Sheng1, ZHANG Hao3, SUN Chengchao1. |
| 1.Department of Thoracic and Cardiovascular Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Department of Emergency, Yueyang First People’s Hospital, Yueyang, 414000; 3.Department of Cardiac Surgery, Fuwai Hospital, Chinese Academy of Medical Sciences, Beijing, 100037 |
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| Cite this article: |
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JIN Peifeng,Wang Yehuan,JIANG Sheng, et al. The different injection site determine the result of bone marrow mesenchymal stem cells transplantation for myocardial infarction[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(7): 485-489.
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Abstract Objective: To determine whether the injection sites of myocardial infarcted area had a various effect on the bone marrow mesenchymal stem cells (BMSCs) survival and efficacy of cell transplantation. Methods: Myocardial infarction (MI) was induced by left anterior descending artery ligation in female rats. 3 weeks after MI, 3×106 bromodeoxyuridine (Brdu) labeled male BMSCs were directly intramyocardial injected into the borderline (BZC group) or central zone (CZC group) of the MI area. In the control groups, the same volume of phosphate buffered saline (PBS) was injected into the borderline (BZP) or the central zone of the myocardial infarction (CZP), respectively. Cell survival at the 24 hours and 4 weeks after transplantation was assayed by quantitative real-time PCR to identify Y chromosome gene. Heart function was evaluated by echocardiography before and after cell transplantation. Ventricular remodeling was analysis by using HE staining with measurement of the expansion index and scar thickness. Cell transplantation induced angiogenesis was tested by VIII factor staining. Results: The detectable transplanted cell survival rate in the BZC group were significantly higher than that of CZC group both at the time of 24 h (15.74%±4.13% vs. 8.2%±2.63%, P<0.01) and 4 weeks (5.57%±1.13% vs. 1.72%±0.41%, P<0.01) after cell transplantation. Compared with the CZC group, improvement of left ventricular ejection fraction (LVEF) and fractional shortening (P<0.05, respectively) were observed in BZC group. Additionally, BMSCs transplanted in the borderline zone also induced augmentation of capillary density than that in the CZC group (P<0.01). Left ventricular remodeling (scar thickness expansion index) in BZC group was significantly improved (P<0.01), and no improved expansion index was identified in CZC group. Conclusion: Borderline area was more suitable than central zone of MI for the survival of the transplanted cells and thereby promoted the angiogenesis and restored the damaged heart function.
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Received: 17 October 2017
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