人巨细胞病毒UL138基因表达对胃癌细胞免疫相关功能的影响

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Abstract Objective: To investigate the effects of HCMV tumor suppressor gene UL138 gene expression on immune-related function in gastric cancer cell. Methods: The recombinant eukaryotic expression plasmid of UL138 gene was constructed and transfected into BGC-823 gastric cancer cells. The expression of UL138 and immunogenic death related protein were verified by Western blot and immunofluorescence. The effect of UL138 expression on gastric cancer cells was analyzed by gene chip, combined with GO, Pathway and other bioinformatics techniques. Fluorescence quantitative PCR was used to further verify the expression of immune related molecules in gastric cancer cells. Results: After being transfected with recombinant plasmid, the expression of UL138 in gastric cancer cells was confirmed by Western blot and immunofluorescence; in addition, Western blot analysis showed that the expression of UL138 up-regulated the expression of ERp57, an immunogenic death associated protein, but did not affect the expression of HSP70. Microarray analysis showed that the expression of UL138 up-regulated 370 genes and down regulated 206 genes. Bioinformatics analysis suggested that these differentially expressed genes were involved in cytoskeletal remodeling, cell adhesion and other pathways involved in gastric cancer and digestive system diseases. Quantitative PCR analysis showed that the expression of UL138 up-regulated the 16 inflammatory related cytokines expression of IL1A, IL6, IL11, IL8, IL1RL1, IL7R, IL13RA2, CCL3L1, CCL5, TNFAIP3, TNFRSF21, VEGFA, CD44, CD55, CD59, CD274 gene and down-regulated CXCL10, IFNAR1 gene. Conclusion: The expression of UL138 was involved in the process of apoptosis of gastric cancer cells, up-regulated expression of IL1A, IL6, IL8 gene of pro-inflammatory cytokines and immune related immunogenicity of death associated protein ERp57.

Key words： stomach neoplasms cytomegalovirus UL138 gene cytokines

Received: 07 December 2017

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	YE Lele
	ZHANG Jiali
	CHEN Wenjing
	SHEN Xian
	HU Changyuan
	DING Ning
	XUE Xiangyang

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I7/474

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