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| Preparation and identification of polyclonal antibodies against M13 phage |
| 1.Department of Clinical Laboratory, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2. Department of Microboilogy Immunology/Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, 325035 |
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TAN Hui1,WANG Bingbing2,LU Xulian2, et al. Preparation and identification of polyclonal antibodies against M13 phage[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(4): 245-247.
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Abstract Objective: To prepare and characterize rabbit-anti M13K07 helper phage polyclonal antibodies. Methods: The helper phage was amplified and used to immunize rabbits to obtain polyclonal antibodies. Begin from the first week, the same immunization schedule of subcutaneous multipoint injection was repeated three times at 2 weeks intervals. Begin from 0 week, take blood serum collection from ear vein every other week. The immunogenicity of the M13K07 helper phage was detected with Dot Immunobinding Assay and indirect ELISA was used to demonstrate the titer of antiserum in the immunized rabbit. Results: It showed that the specific rabbit antibody against M13K07 helper phage could combined with M13K07 with Dot Immunobinding Assay. Indirect ELISA analysis showed that the helper page can stimulate high levels specific IgG antibody. Along with the increase of immunization times, antibody levels keep rising, reached the peak at the 6th week. The titer of the polyclonal antibody was approximately1:3 200. Conclusion: In this study, polyclonal antibodies against this M13K07 helper phage is successfully generated and such helper phage polyclonal antibodies is important reagent for functional analysis for phage.
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Received: 23 October 2013
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2014, Vol. 44 
