siRNA靶向沉默成纤维细胞激活蛋白α对胶质瘤细胞U87增殖的影响

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Abstract Objective: To explore the effect of small interfering RNA (siRNA) -mediated fibroblast activation protein alpha knock-down on proliferation of glioma cell line U87. Methods: The experiment is divided into Blank, NC and siRNA. Negative control or siRNA were transfected into U87cells by Lipofectamine 2000. After transfection, protein level of target gene FAP-α, Bcl-2 and caspase-3 were detected by Western blot, mRNA level of PCNA and FAP-αwere detected by Real-time PCR. The proliferation ability of NC group and siRNA group of cells were analyzed by CCK8 assay. Results: ①Forty-eighth after transfection, mRNA and protein expressions of FAP-αin siRNA group were remarkably reduced as compared with those in NC and Blank group (P<0.05); Compared with NC group, the expression of caspase-3 was higher (P<0.05), while that of Bcl-2 was lower (P<0.01). The expression of PCNA mRNA was lower than NC group (P<0.01). ②The OD450 value of siRNA group was significantly lower than that in the NC group 24, 48, 72 and 96 h after transfection (P<0.05). Conclusion: siRNA-mediated fibroblast activation protein alpha knock-down can inhibit the proliferation of U87 glioma cells and promote apoptosis of glioma cells.

Key words： glioma small interfering RNA fibroblast activation protein alpha cell proliferation function

Received: 28 October 2013

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	CHEN Yuan
	WANG Wei
	TU Ming
	WU Zhebao
	ZHENG Weiming.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I4/241

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