|
|
|
| Experimental study of microporous acellular dermal matrix and mesenchymal stem cells composite grafttor remodeling the cutaneous defect of nude mice |
| WAN Li1．LU0 Xu2．xIN Guohua3．LI Anle4．ZENGTaofang5．XIA Weidong2．LI Xiaokun6．LlN Cai2 |
1．Department ofPathology,the First Affiliated Hospital of Wenzhou Medical College，Wenzhou．325000；2．Department ofBurn．the Ftnl Affiliated Hospital of Wenzhou Medical College．Wenzhou．325000；3．Department ofBurn。the First Affiliated Hospital ofNanchang University,Nanchang，330000；4．ExperimentalAnimal Center,Wenzhou Medical Coltege，Wenzhou，325035；5．Department of Burn．the People's Hospital of Yichum City,Yichun，336000；6．School ofPharmacy,Wenzhou Medical College．
Wenzhou，325035 |
|
|
|
Abstract Objective：To investigate the healing effect and the remodeling practicability of skin function of the composite graft consisting of laser micropore porcine acellular dermal matrix(LPADM)and mesenchymal stem cells(MSCs)on full—thickness skin wounds．Methods：The composite grafts were prepared by seeding the MSCs on the LPADM(MSCs—LPADM)or the normal porcine acellular dermal matrix(MSCs—PADM)．0ne fullthickness wounds(2 cm x 2 cm)were created on each back of l 8 nude mice and were randomly divided into 3groups(n=6)．Then the MSCs—LPADM were transplanted synchronously with the split—thickness autograft on
the wounds(group A)．The mice treated with LPADM(group B)or MSCs—PADM(groups C)were considered as control．The gross wound healing status was examined．Tissue samples were collected at day 3，7 and 14 post—operation for histological examination and transmission electron microscopy(TEM)obeservation．Results：H&E staining showed the appearance of the cavity—like vascular structure in group A and B at day 3 post—operation，but not in group C．At day 7，the grafts of A and B groups survived well and new vessels can be seen evidently in the micropores．However，grafts necrosis occurred in group C with pallor or black tissue．At day 14，all the grafts survived in A and B groups and abundant blood vessels in the micropores of the matrixs were observed as well，while in group C，no blood vessel appeared and all the grafts went through necrosis and exfoliation．Through TEM observation，we found newly generated unmyelinated nerve—like structure in group A with visible sebaceous gland—like cells in the superficial layer and single sebaceous gland—like cells in the deep layer of the demal matrix，which can not be seen in group B and C．Conclusion：The composite graft(MSCs—LPADM)has better healing effect and provides a good microenvironment for the exogenous hMSCs to proliferation and differentiation，thus may have agreat potential for use in wound reconstruction in the future．
|
|
|
| |
|
|
|
|
|
|
