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| The role of caveolin．1 and p421p44MAPK in the airway remodeling of asthmatic rats and the influence of roxithromycin on its expression |
| WANG Ruili1,CHEN Huijun2．DAI Yuanrong1, WANG Zhonghai2,YANSunshun1,ZENG Weixian3． |
| 1.Department ofRespiratory Medicine，the Second Affiliated Hospital of WenzhouMedical College，Wenzhou，325027；2．Department ofRespiratory Medicine，Jinhua Municipal Central Hospital，Jinhua．321000；3．Department ofEmergency,the Second Affiliated Hospital of Wenzhou Medical College，Wenzhou，325027 |
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Abstract objective：To explore the role of caveolin一1 and p42／p44MAPK in the airway remodeling of asthmatic rats and the intervention of Roxithromycin．Methods：Thirty—two healthy.male Sprague—Dawley rats were randomly divided into four groups averagely：control group(group C)，asthmatic group(group A)，Dexamethasone group(group D)and Roxithromycin group(group R)．Asthmatic rats model was duplicated by the sensitization and provocation with ovalbumin．The total bronchial wall thickness(Wat／Pbm)and the bronchial smooth muscle thickness(Wam／Pbm)were measured with image—pro plus 6．0．The protein expressions of caveolin一1 and p-p421 p44MAPK in the airway smooth muscle were measured with Western blot．Results：Wat／Pbm，Wam／Pbm of group A were significantly higher than those ofgroup C(P<0．01)，those ofgroup D and group R were lower than those of group A(P<0．01)，nevertheless，higher than those of group C(P<O．05)．The expression of caveolin一1 in group A was significantly lower than that of group C(P<O．01)，that of group D and group R were significantly higher than that of group A(P<0．01)，but decreased compared with group C(P<0．05)；the expression of p-p42／p44MAPK in groupA significantly increased compared with group C(P<0．01)，that of group D and group R were significantly decreased than that of group A(P<0．01)，but still increased compared with group C(P<0．01)．There were significantly negative correlation between Wam／Pbm and the protein expression of caveolin—l(r=一0．893，P<0．01)．There were significantly positive correlation between Wam／Pbm and the expression of P—D42／I：144MAPK measured with Western blot(r=-0．918．P<0．01)．There was significantly negative correlation between the expressions ofcaveolin一1 and P—p42／p44MAPK measured with Western blot(r=．0．873，P<O．0 1)．Conclusions：There may be some connections between caveolin一1 and 042／044MAPK signaling in airway remodeling of asthmatic rats．and Roxithromycin maybe inhibits airway remodeling partly through caveolin一1 and p42／p44 MAPK signaling pathway．
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