尾加压素II受体拮抗剂对高肺血流性肺动脉高压大鼠肺组织TGF-β1表达的影响

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Abstract Objective: To explore the impact of urotensin-II (UII) on the expressions of transforming growth factor β1 (TGF-β1) in rat model of pulmonary arterial hypertension triggered by high pulmonary blood flow. Methods: Sixty male SD rats were included and randomly divided into pulmonary hypertension model group (Group S, n=20), sham group (Group C, n=20), urotensin II inhibitor group (Group M, n=20). The pulmonary hypertension model was established after surgical connection of the common carotid artery and jugular vein in rats. Four weeks, eight weeks and twelve weeks later, 5 rats were selected from each group to measure the right ventricular pressure. Twelve weeks later, 5 rats from each group were sacrificed to obtain specimens of lung tissue. Western blot was used to detect the expression of UII and TGF-β1 protein in lung tissue. The content of TGF-β1 mRNA was detected via RT-PCR. One-way analysis of variance and Pearson correlation analysis were used to analyze the data. Results: For the pulmonary artery pressure, Group S reached a higher level compared with Group C (P<0.05), and the level of Group M was significantly decreased when compared with Group S (P<0.05). Twelve weeks later, the content of UII protein in Group S was remarkably increased (P<0.05) while in Group M was relatively decreased (P<0.05) compared with Group C. Twelve weeks later, the expression of both TGF-β1 mRNA and protein in Group S was higher than Group C (P<0.05), but in Group M it were significantly decreased (P<0.05). The expression of UII protein was positively associated with pulmonary artery pressure and TGF-β1 mRNA and protein expression. Conclusion: The down-regulation of UII reduces the expression of TGF-β1, which may ultimately alleviate the pulmonary hypertension in the rat model.

Key words： rats urotensin II transforming growth factor-β1 high pulmonary blood flow pulpulmonary arterial hypertension

Received: 30 December 2017

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	ZHANG Songyue
	REN Yue
	HE Yuee
	LI Hao
	WU Rongzhou

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I6/441

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