胚胎干细胞分泌因子增强AML-12细胞抗凋亡能力

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Abstract Objective: To investigate whether the embryonic stem cell secreted cytokines can enhance AML-12 cell anti-apoptotic ability in extreme cell culture conditions. Methods: AML-12 cells were respectively treated with embryonic stem cells complete media (CON-M) or embryonic stem cells conditioned media (ESC-M) and cultured in extreme cell culture conditions. Cells viability was detected by MTT assay. Mitochondrial energy metabolism including oxygen consumption rate and extracellular acidification rate were measured using a Seahorse metabolic flux analyzer. Results: MTT assay indicated that AML-12 cells treated with ESC-M had stronger anti-apoptotic abilities in extreme cell culture conditions. Results from the Seahorse metabolic flux analyzer showed that ESC-M could increase AML-12 cells maximal respiration capacity, spare respiration capacity as well as glycolytic capacity. Conclusion: The embryonic stem cells secreted cytokines enhance AML-12 cells anti-apoptotic abilities in extreme cell culture conditions.

Key words： embryonic stem cell cell survival hepatocyte metabolism AML-12 cells

Received: 18 January 2018

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	LIN Wu
	HUANG Zhaoshuai
	BAO Miao
	CHEN Xiaoming.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I6/408

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