稳定沉默黏着斑激酶结肠癌细胞株SW620的构建

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Abstract Objective: To establish the FAK stable silencing SW620 cells. Methods: For production of lentiviruses, 293FT cells were transfected by Turbo transfect, using pLL3.7 FAK and negative pLL3.7, lentivirus-containing media were harvested and used to infect SW620 cells as experimental group and control group, SW620 cells didn抰 infect any lentiviruses as unsettled group. Infection efficiency was obtained by the expression of GFP, and maintained as a pool population. Expression of FAK was detected by RT-PCR and Western blot. Results: Lentiviruses were successfully producted with over 90% infecting rate; cells stable expressing GFP. Expression of FAK were significantly reduced by RT-PCR and Western blot. Conclusion: FAK stable silencing SW620 cells are established.

Key words： focal adhesion kinase RNA interference SW620 colorectal neoplasms metastasis

Received: 18 March 2013

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	HONG Weiwen1
	SU Guoqiang2
	YING Hong'an3
	LIN Feng1
	LIANG Weidong1.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I3/201

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