YAP1 基因敲除对胰腺癌L3.6细胞增殖及迁移能力的影响

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Abstract Objective: To investigate the effects of YAP1 on proliferation and migration in pancreatic cancer cells. Methods: CRISPR-Cas9 technique was used to knockout the YAP1 gene in human pancreatic cancer L3.6 cells, and Western blot was used to verify it. The proliferation ability of L3.6 cells before and after YAP1 knockout was detected by MTT colorimetry and EdU flow cytometry. The migration ability was measured by cells scratch and Transwell assay. Results: The L3.6 stable cell lines of YAP1 knockout (YAP1-KO) were successfully obtained. Compared with wild type L3.6 cell lines, the proliferation and growth abilities of YAP1-KO cells were significantly decreased, and the invasion ability of cells was also decreased. Conclusion: Knockout of YAP1 gene can significantly reduce the proliferation and migration abilities of pancreatic cancer L3.6 cells, which might provide a new potential target for targeted therapy of pancreatic cancer.

Key words： Yes-associate protein 1 human pancreatic cancer cells Cas9 technology proliferation migration

Received: 15 January 2018

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	CHEN Qianjie
	ZHANG Jinsan
	GUO Qiang
	DONG Jinglai
	GUO Lisha
	LI Xiaokun

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I6/391

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