丁香精油和茉莉精油对抑郁症小鼠的治疗效果及其作用机制

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Abstract Objective: To study the effects of odorants on the treatment of depression and their mechanisms. Methods: Forty-five C57BL/6J mice with similar in multiple physiological indices were selected. Eight of them were randomly picked out as norm group and the others were stimulated by the depression modeling methods: chronic unpredictable mild stress model (CUMS) and solitary rearing. After 28 days, 32 mice were screened and randomly divided into 4 groups. Three groups were treated respectively by using clove oil (clove group), jasmine oil (jasmine group) and distilled water (distilled water group) in atomization. The treatments were executed 3 times every day and one hour per time, lasting 28 days. The last group (depression group) and the normal group were not given treatment and normally reared for 28 days. Subsequently, multiple indices involve weight tail suspension test (TST) forced swim test (FST) and the serum concentration of 5-HT of mice and the expression levels of olfactory receptors (MOR11, MOR1, MOR124, MOR16, MOR378, MOR544, MOR19, MOR744, MOR63, MOR148, MOR9, MOR151, MOR15) of mice in all groups were tested. Results: The depression model of mice was successfully established after 28 days. After treatment, there were no significant difference in the indices of weight tail suspension test (TST) forced swim test (FST) and the serum concentration of 5-HT of mice between clove group and normal group, but the four indices decreased significantly, compared with the depression group and the distilled water group. However, all the indices except of the weight of jasmine group were significantly increased compared with normal group. There was no significant difference in other indices compared with the depression group and distilled water group except that the weight increased significantly, There were no significant differences in the four indices between distilled water group and depression group. The RT-qPCR results showed that the 12 of 13 olfactory gene expression levels in mice significantly decreased after depressed. After treatment, compared with the normal group, in clove group, 8 genes (MOR11, MOR124, MOR15, MOR16, MOR378, MOR63, MOR744, MOR1) expression levels increased significantly (P<0.05), 3 genes (MOR19, MOR151, MOR544) had no significant difference (P>0.05), the expression level of 2 genes (MOR148, MOR9) were significantly decreased (P<0.05); In jasmine group, only 2 genes (MOR744, MOR1) expression level was significantly increased (P<0.05), 3 genes (MOR11, MOR63, MOR151) had no significant difference (P>0.05), the expression levels of other genes (MOR124, MOR15, MOR16, MOR19, MOR378, MOR544, MOR148, MOR9) decreased significantly (P<0.05); in distilled water group 4 genes (MOR11, MOR63, MOR744, MOR1) expression levels increased significantly (P<0.05), 4 genes (MOR124, MOR16, MOR378, MOR151) expression level showed no significant difference (P>0.05), and the expression level of the other genes (MOR15, MOR19, MOR544, MOR148, MOR9) decreased significantly (P<0.05). There were no significant differences in the remaining genes in each group. Conclusion: Clove oil is highly effective on depression, but jasmine oil and distilled water are rarely. Furthermore, the effect of odorants on depression is closely related to olfactory gene expression levels.

Key words： depressive disorder clove oil jasmine oil olfactory receptor genes chronic unpredictable mild stress polymerase chain reaction mice

Received: 12 October 2017

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	LIN Huiyue
	WAN Xinlong
	DU Yongjun
	XIE Hongli.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I5/330

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