人脂肪间充质干细胞对宫颈癌细胞的趋化性

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Abstract Objective: To investigate the chemotaxis of human adipose tissue derived mesenchymal stem cell (hATMSC) to human cervical cancer cells and to provide experimental basis for the development of hATMSC as a target vehicle for the treatment of cervical cancer. Methods: Primary hATMSC from subcutaneous fat of patients undergoing cesarean section was isolated. hATMSC was induced by osteogenic and adipogenic differentiation medium, and using Alkaline Phosphatase, Oil Red O staining respectively to verify the differentiative capacity. The expression of surface antigen and OCT-4 mRNA were detected by flow cytometry and qRT-PCR, respectively. The migration ability of hATMSC to cervical cancer squamous cells SiHa, MS751, C33-A, CaSki and adenocarcinoma cell Hela was detected by Transwell assay. Results: Having been incubated in osteogenic and adipogenic differentiation medium for 14 days, hATMSC was positive by Alkaline Phosphatase and Oil Red O staining. The hATMSC was positive for CD90, CD44, CD105 and HLA-ABC, but negative for CD34, CD45, HLA-DR. OCT-4 mRNA was highly expressed in hATMSC. hATMSC had high motility to migrate to cervical cancer cells, and the migration to C33-A was the strongest among them. Conclusion: hATMSC was successfully isolated and cultured, with the characteristics of stem cells having multi-directional differentiation potential. hATMSC has obvious chemotaxis to different cervical cancer cells, suggesting that hATMSC might be used as a potential carrier for targeting therapy of cervical cancer.

Key words： adipose mesenchymal stem cell uterine cervical neoplasms tropism

Received: 18 December 2017

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	ZHENG Lihong
	LIU Yi
	ZHU Xueqiong.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I5/325

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