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1H NMR based metabonomics analysis of serum from mice with diabetic nephropathy |
WEI Tingting1, ZHAO Liangcai2, GAO Hongchang2. |
1.Laboratory Animal Research Center, Wenzhou Medical University, Whenzou, 325035; 2.School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035 |
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Cite this article: |
WEI Tingting,ZHAO Liangcai,GAO Hongchang.. 1H NMR based metabonomics analysis of serum from mice with diabetic nephropathy[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(5): 313-318.
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Abstract Objective: To study the characteristic metabolites and the related pathways in serum of db/db
diabetic mice by 1H nuclear magnetic resonance (1H NMR)-based metabonomics with correlative analysis. Methods: The urine and serum of eight db/db mice and nine C57BL/6J mice were collected at week 17. Then the blood glucose, urine creatinine (UCr) and urinary albumin to creatinine ratio (UACR) were measured. The characteristic alterations in serum metabolites were explored by the projection to latent structure discriminant analysis based on the 1H NMR spectra. The correlation between UACR, UCr and serum metabolites was performed by Pearson correlation analysis. Results: At 17-week, db/db mice showed apparent obesity, with the weight more than twice that of wild type mice. The blood glucose and UACR of db/db mice were also doubled compared with wild type mice, but the UCr decreased. It was demonstrated that the db/db mice showed typical symptoms of diabetic nephropathy at week 17. The metabolic profile of the two kinds of mice showed clear differences along the PC1 direction. Compared with wild type mice, pyruvate, lactate, citrate, 3-hydroxybutyrate, acetoacetate, glycine, glutamine, tyrosine, phenylalanine, creatine, choline were reduced, LDL/VLDL and isoleucine/leucine were increased in db/db mice (P<0.05). UACR showed a positive correlation with choline in the serum of db/db mice (r=0.717, P<0.05). Conclusion: In the pathogenic process of diabetic nephropathy, the tricarboxylic acid cycle, glycolysis and lipid metabolic pathways are suppressed and glycogenesis is elevated. Choline may be a potential biomarker of diabetic nephropathy.
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Received: 25 January 2018
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