雌激素对人乳头状瘤病毒16型E6基因转染人永生化表皮细胞效率的影响

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Abstract Objective: To determine the influence of 17-β-estradiol (E2) on human papilloma virus 16 E6 gene transfecting HaCat cell line and the possible mechanism. Methods: The HaCat cell line was incubated with E2 of varies concentration (10-5, 10-6, 10-7, 10-8, 10-9, 0 mol/L) .The cell number was counted for 7 days to draw a growth curve. The cell viability was determined by MTT method. Flow cytometry was used to detect cell cycle distribution and BrdU to determine the time duration of each cell cycle phase. Then, the E2 treated cells was transfected by the plasmid containing HPV E6. The expression of E6 mRNA was determined by quantitative realtime-PCR. Results: Compared with the control, at the concentration between 10-7-10-5 mol/L, E2 was observed to increase the number of HaCat cells, reduce the doubling time of cell number and increase the cell viability and E6 mRNA expression after transfection (P<0.05). Conclusion: E2 increased the cell growth and viability, enhanced the transfection efficiency of E6 on HaCat cells. The mechanism may be related to the acceleration of cell growth and cell viability.

Key words： human papilloma virus 16 E6 gene 17-β-estradiol estrogen receptor HaCat cell line

Received: 02 November 2017

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	LU Xiaosheng
	CHEN Jing
	ZHAO Junzhao
	LYU Jieqiang

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I4/280

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