3-甲基腺嘌呤对人耐药大肠癌SW480细胞增殖及自噬活性的影响

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Abstract Objective: To investigate the effects of 3-methyladenine on chemotherapy sensitivity of 5-Furesistant colon cancer cell line SW480 and reversing the 5-Furesistant in colon cancer. Methods: Stepwise 5-Fu selection was used to establish the 5-Fu resistant colon cancer cells (100μg/L). The cells were dealed with 3-methyladenine (10μmol/L) fistly and 5-Fu (200μg/L) for 24 hours. Cell proliferation inhibition rate was measured with CCK-8. Apoptosis rate was detected with flow cytometer. Autophagic vacuoles in cells were observed by means of transmission electron microscopy and monodansylcadaverine (MDC). Results: The inhibition rate of 3-methyladenine in the 5-Fu resistant cell autophagy activity was 89.7%, cell proliferation was 9.6%, but apoptosis rate was just 5.7%, were obviously higher than that in 5-Fu resistant cells (P<0.05). After dealing with 3-methyladenine(10μmol/L) fistly and 5-Fu (200μg/L) for 24 hours, the level of cell proliferation inhibition rate was 71.6%±2.9% and the apoptosis rate was 36.6%±2.9%, while dealing with 5-Fu (200μg/L) for 24 hours, the level of cell proliferation inhibition rate was 23.6%±2.9% and the apoptosis rate was 10.3%±2.5%. After dealing with 3-methyladenine, the cell proliferation inhibition rate and apoptosis rate were markedly higher than that of 5-Fu only. Conclusion: The anticancer capacity of 3-methyladenine in colon cancer is weak, but it can reverse the 5-Fu-resistant in colon cancer partly, 5-Fu-induced apoptosis in 5-Fu resistant colon cancer cells may be enhanced by the inhibitor of autophagy.

Key words： colonrectal neoplasms autophagy 3-methyladenine fluorouracil drug resistance

Received: 25 October 2013

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	HU Wanle
	WANG Lingqin
	CHEN Suping
	CHEN Rong.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I3/189

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