长链非编码RNA FENDRR在子宫颈癌中的作用

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Abstract Objective: To investigate the effects of long non-coding RNA FENDRR in cervical cancer. Methods: Cervical cancer cells were transfected with siRNA that specifically targeting FENDRR. QRT-PCR was used to verify the transfection effect of siRNA. Proliferation and motility capacities of cervical cancer cells were demonstrated by CCK-8 and Transwell assays, respectively. Tumorigenesis in nude mice was performed to identify the proliferation ability of cervical cancer cell in vivo. Results: With siRNA transfection, the expression levels of FENDRR in SiHa and HeLa were significantly reduced from (1.013±0.120), (1.001±0.025) to (0.226± 0.024), (0.099±0.010) (P<0.01). After transfecting with siRNA targeting FENDRR, the capacity of proliferation was significantly enhanced in cervical cancer cells (P<0.01), as well as migration and invasion abilities (P<0.01). The tumorigenesis in nude mice was obviously improved by interfering FENDRR expression in HeLa. Compared with the control group, the tumor volume increased from (853.2±59.4) mm3 to (1 155.0±51.5) mm3 (P<0.01), the tumor weight increased from (0.544±0.053) g to (0.814±0.061) g (P<0.05). Conclusion: Knockdown the expression of FENDRR in cervical cancer cells significantly enhanced their proliferation, migration as well as invasion abilities in vitro, and improved their tumorogenesis in vivo, which indicated that FENDRR may play an important inhibitory role in the progression of cervical cancer.

Key words： long non-coding RNA FENDRR gene uterine cervical neoplasms RNA, small interfering cell proliferation cell movement tumorigenesis

Received: 24 July 2017

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	LAN Xiaoxiao
	ZHOU Zhiyang
	XU Xinxin
	WU Xueqing

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I4/262

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