微小RNA-155反义寡核苷酸对乳腺癌MDA-MB-157细胞及裸鼠移植瘤的作用

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Abstract Objective: To explore the effects of antisense oligonucleotide targeting miR-155 on proliferation and apoptosis in MDA-MB-157 cells and the growth of implanted tumor in nude mice. Methods: 2扥me modified antisense oligodeoxynucleotide targeting miR-155 was synthesized and then transfected into MDA-MB-157 cells by LipofectamineTM 2000. Transfection efficiency was detected by laser confocal microscope. Real-time RT-PCR method was used to detect the expression of miR-155. Cell counting Kit-8 (CCK-8) assay was used to detect the proliferation of MDA-MB-157 cells and cell apoptosis rate was measured by flow cytometry. The effects of miR-155 Antisense oligonucleotide (miR-155 ASO) on the transplanted tumor were assessed in nude mice. Results: Transfection efficiency detected by laser confocal microscope was higher than 80%. The level of miR-155 expression was significantly decreased (P<0.05) in the cells transfected with miR-155 ASO, compared with that in cells transfected with negative control. After being transfected with miR-155 ASO, the viability of MDA-MB-157 cells reduced greatly (P<0.05) and the number of apoptotic cells increased significantly. Additionally, miR-155 ASO inhibited the growth of transplanted tumor in vivo (inhibition ratio=52.98%) and significantly increased the expression of caspase-3. Conclusion: miR-155 ASO can effectively down-regulate the expression of miR-155, induce cell apoptosis and inhibit cell proliferation and tumor growth by increasing the expression of caspase-3. These findings provide the experimental evidence for using miR-155 as a therapeutic target of breast carcinoma.

Key words： breast neoplasms microRNA-155 antisense oligonucleotide MDA-MB-157 cells nude mice

Received: 19 March 2013

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	ZHENG Shurong
	GUO Guilong
	HUANG Qidi
	HUANG Duping
	YOU Jie
	HUANG Guanli.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I3/183

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