FISH技术检测hTERC基因表达在宫颈病变诊治中的意义

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Abstract Objective: To investigate the clinical significance of human telomerase RNA gene component (hTERC) detection in exfoliated cervical cells by fluorescence in situ hybridization (FISH) technique. Methods: hTERC gene amplification was tested by FISH in 235 patients at the First Affiliated Hospital of Wenzhou Medical University from December 2014 to October 2016. Retrospective analysis was made to explore the relationship between hTERC gene expression and the severity of histological and cytological abnormality. The association between hTERC gene amplification and postoperative pathological upgrade was analyzed in 61 patients who chose cervical conization surgery after FISH detection. Results: With the severity of cytological abnormality, hTERC gene abnormal amplification rate increased significantly (P<0.05). With the severity of histological abnormality, hTERC gene amplification rate and abnormal amplification rate increased significantly (P<0.05). The pathological upgrade rate after cervical conization surgery in patients with abnormal hTERC gene amplification rate was significantly higher than those with normal amplification rate (P<0.05). Conclusion: With the severity of cervical lesions, the expression of hTERC gene amplification rate increased. The pathological upgrade rate in patients with abnormal hTERC gene amplification rate was more likely to be higher than those with normal amplification rate. These results suggest that the hTERC gene detection with FISH technique has predictive value in the progression of cervical lesions.

Key words： hTERC fluorescence in situ hybridization cervical intraepithelial neoplasia telomerase

Received: 23 June 2017

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	CHEN Lulu
	WANG Yuli
	ZHOU Qing
	ZHOU Kai
	ZHU Hua
	HU Yan.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I3/207

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