CYP1B1与北方地区汉族人群喉癌易感性多基因关联分析

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Abstract Objective: To explore the association between functional polymorphisms and combined effect in the CYP1B1 (CYP1B1*2 142 C/G, CYP1B1*2 355 G/T, CYP1B1*3 4326 C/G) enzyme genes and the susceptibility of laryngeal cancer in northern Han Chinese. Methods: A case-control study was performed for 200 northern Han Chinese patients diagnosed with laryngeal cancer and 200 healthy control individuals. The genetic polymorphisms of CYP1B1*2 142 C/G, CYP1B1*2 355 G/T, CYP1B1*3 4326 C/G were examined for the individuals by multi-PCR and Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF MS) approach. The association of these genetic polymorphisms, smoking, drinking, and the combinations of these factors with the susceptibility of laryngeal cancer was statistically evaluated. Results: The frequencies of CYP1B1*2 355 G/T, CYP1B1*3 4326 C/G gene polymorphisms in the patient cohort were significantly different with those in the control cohort (P<0.001, P=0.007 respectively). Compared to the control cohort, a higher risk laryngeal cancer was observed for the carriers of variant genotypes of CYP1B1*2 355T(adjusted OR=2.281,95%CI: 1.142-3.516, P<0.001) and the mutation allele CYP1B1*3 4326G carriers had lower laryngeal cancer risk (adjusted OR=0.571, 95%CI: 0.370-0.882, P=0.011). Polymorphisms at these three loci presented a joint effect. The haplotype C142T355C4326 was associated with a significantly higher laryngeal cancer risk (adjusted OR=3.180, 95% CI:1.760-5.746, P<0.001) compared to the haplotype C142G355C4326. The adjusted ORs for the individuals with GT/TT genotype at CYP1B1*2 355 G/T were 2.080 (95% CI: 0.742-5.830, P<0.001) and 6.322 (95% CI: 2.541-15.725, P<0.001) respectively for the non-smokers and smokers. Conclusion: Genetic polymorphism of CYP1B1 is associated with risk of laryngeal cancer. CYP1B1*2 355T increase the laryngeal cancer risk and CYP1B1*3 4326G reduces the risk. The association between CYP1B1*2 142C/G polymorphism with the risk of laryngeal cancer dose not found. These polymorphisms showed a joint effect on the susceptibility to laryngeal cancer, presenting as higher risk with more risky factors included. Environmental factors, smoking and drinking, showed a non-collaborative effect with two risky genetic factors.

Key words： laryngeal neoplasm polymorphism interaction SPNs CYP1B1

Received: 09 November 2013

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	LIN Faming1
	JIN Jianhua2
	XUE Jing3
	BAO Qiyu4
	LIU Chaobing5
	NI Liyan1.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I3/177

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