白藜芦醇对肠易激综合征大鼠脑源性神经营养因子系统表达的影响

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Abstract Objective: To investigate the effect of resveratrol on depression- and anxiety-like behaviors, intestinal motility and visceral sensitivity in rats with irritable bowel syndrome. Methods: Forty-two SD rats were randomly divided into normal control group, chronic-acute combined stress (CACS) group, resveratrol group with different doses (10, 20 and 40 mg/kg), fluoxetine group and diazepam group. Twenty-two day CACS was used to established CACS model. The depression- and anxiety-like behaviors were evaluated by forced swimming test and marble-burying task. Intestinal motility and visceral sensitivity were measured by intestinal motility test and AWR test. The levels of brain-derived neurotrophic factor (BDNF) in hippocampus, frontal cortex, ileum and colon were measured by Western blot. Results: Compared with the normal group, the duration of immobility time of forced swimming and the number of marble buried was significantly increased (P<0.01 and P<0.01, respectively); the number of fecal output (P<0.01) in the intestinal motility test and the abdominal withdrawal reflex (AWR) score were also increased in CACS group (20 mmHg: P<0.05; 40 mmHg: P<0.05; 60 mmHg: P<0.01; 80 mmHg: P<0.01). The expression of BDNF in hippocampus (P<0.01) and the frontal cortex (P<0.01) were decreased, while the expression of BDNF in colon (P<0.01) and ileum (P<0.05) were increased in the CACS group, as compared with the normal control group. After 22-day treatment with resveratrol, the above data have been improved. Conclusion: Resveratrol ameliorated the behavior of IBS rats, which is likely to be related to the regulation of BDNF expression in the brain and gut.

Key words： resveratrol irritable bowel syndrome brain-derived neurotrophic factor chronic acute combined stress rats

Received: 17 October 2017

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	ZHANG Jianbo
	ZHU Naping
	CHEN Jie
	LIU Kaiping
	YE Han
	XU Wen
	PAN Jianchun.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I3/178

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