基因沉默整合素连接激酶对滋养细胞生物学功能的影响

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Abstract Objective: To investigate the effects of integrin linked kinase (ILK) on the proliferation, migration and invasion of human extravillous trophoblastic cells (TEV-1). Methods: ILK-interferenced RNA was constructed and transfected into human TEV-1 cells, which was divided into experimental group (KD) and negative control group (NC); qRT-PCR and Western blot were used to detect the expression of ILK mRNA and protein. MTT assay, Transwell assay and Scarification test were used to detect the cell proliferation, migration and invasion. Results: Silenced ILK gene significantly reduced the ILK mRNA and protein expression levels of the TEV-1 cell (P<0.05), indicating that the transfection was successful. Silenced ILK gene significantly inhibited the proliferation, invasion (P<0.01) and migration of TEV-1 cells (P<0.05). Silenced ILK gene significantly decreased the mRNA and protein levels of MMP-9 and p-GSK3β in TEV-1 cells (P<0.01). Conclusion: These findings indicate that silenced ILK gene perturbs proliferation, migration and invasion may through down-regulated GSK3β and MMP-9 in TEV-1 cells.

Key words： integrin linked kinase RNA interference extravillous trophoblast cell proliferation cell movement cell invasion

Received: 13 November 2017

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	LIN Yanyan
	LIAO Tingting
	JIN Jiaxi
	CHEN jiajia
	HUANG Xianping
	XIANG Huiqiu
	DONG Ke
	XU Zhangye.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I3/168

[1] RED-HORSE K, ZHOU Y, GENBACEV O, et al. Trophoblast differentiation during embryo implantation and formation of the maternal-fetal interface[J]. J Clin Invest, 2004, 114(6): 744-754.
[2] BISCHOF P, IRMINGER-FINGER I. The human cytotrophoblastic cell, a mononuclear chameleon[J]. Int J Biochem Cell Biol, 2005, 37(1): 1-16.
[3] WARRINGTON J P, GEORGE E M, PALEI A C, et a1. Recent advances in the understanding of the pathophysiology of preeclampsia[J]. Hypertension, 2013, 62(4): 666-673.
[4] CIANTAR E, WALKER J J. Preeclampsia, severe preeclampsia and hemolysis, elevated liver enzymes and low platelets syndrome: What is new?[J]. Womens Health (Lond), 2011, 7(5): 555-569.
[5] BOULTER E, VAN OBBERGHEN-SCHILLING E. Integrin-linked kinase and its partners: A modular platform regulating cell-matrix adhesion dynamics and cytoskeletal orga-nization[J]. Eur J Cell Biol, 2006, 85: 255-263.
[6] LEGATE K R, MONTANEZ E, KUDLACEK O, et al. ILK, PINCH, and parvin: the tIPP of integrin signaling[J]. Nat Rev Mol Cell Bio, 2006, 7(1): 20-31.
[7] CHOU C C, CHUANG H C, SALUNKE S B, et al. A novel IF-1alpha-integrin-linked kinase regulatory loop that facilitates hypoxia-induced HIF-1alpha expression and epithelial-mesench-ymal transition in cancer cells[J]. Oncotarget, 2015, 6(10): 8271-8285.
[8] YAN Z, YIN H, WANG R, et al. Overexpression of integrin-linked kinase (ILK) promotes migration and invasion of colorectal cancer cells by inducing epithelial-mesenchymal transition via NF-kappaB signaling[J]. Acta Histochem, 2014, 116(3): 527-533.
[9] ZHENG Q, XU Y, LU J, et al. Emodin inhibits migration and invasion of human endometrial stromal cells by facilitating the mesenchymal–epithelial transition through targeting ILK[J]. Reprod Sci, 2016, 23(11): 1526-1535.
[10] ELUSTONDO P A, HANNIGAN G E, CANIGGIA I, et al. Integrin-Linked Kinase (ILK) is highly expressed in first trimester human chorionic villi and regulates migration of a human cytotrophoblast-derived cell line[J]. Biol Reprod, 2006, 74(5): 959-968.
[11] GIL D, CIOŁCZYK-WIERZBICKA D, DULIŃSKA-LITEWKA J, et al. Integrin-linked kinase regulates cadherin switch in bladder cancer[J]. Tumor Biol, 2016, 37(11): 15185-15191.
[12] LUO L, LIU H, DONG Z, et al. Small interfering RNA targeting ILK inhibits EMT in human peritoneal mesothelial cells through phosphorylation of GSK-3β[J]. Mol Med Rep, 2014, 10(1): 137-144.
[13] MATSUI Y, ASSI K, OGAWA O, et al. The importance of integrin-linked kinase in the regulation of bladder cancer in-vasion[J]. Int J Cancer, 2012, 130(3): 521-531.