姜黄素对APP/PS1双转基因小鼠学习记忆及海马炎症信号通路的影响

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Abstract Objective: To investigate the effect of feeding curcumin on learning and memory ability, amyloid-β (Aβ) and the protein expression of high mobility group box protein-1 (HMGB1), receptor for advanced glycation endproducts (RAGE), Toll-like receptors 4 (TLR4), nuclear factor kappa B (NF-κB) p65 in the hippocampus of amyloid precursor protein / presenilin 1 (APP/PS1) double transgenic mice. Methods: Male transgenic mice (n=20) were randomly divided into model group (M group, n=10) and treatment group (T group, n=10), the littermate wild-type male mice (n=10) were used as control (C group, n=10). The 4-month-old mice in T group were fed with curcumin diet (100 mg•kg-1•d-1), M group and C group were fed with normal diet. Morris water maze was applied to test the learning and memory ability for all 9-month-old mice. Aβ and the protein expression of HMGB1, RAGE, TLR4, NF-κB p65 in the hippocampus were determined by immunohistochemistry and Western blot respectively. Results: Compared with group C, the mice in group M had longer escape latencies (P<0.05) and the average number across platform was decreased (P<0.05) in Morris water maze test, the number of Aβ and the value of IOD was increased in CA1 region and DG region of hippocampus (P<0.05), while the protein expression of HMGB1, RAGE, TLR4, NF-κB p65 (P<0.05) was significantly increased. Compared with group M, the mice in group T had shorter escape latencies (P<0.05) and the average number across platform was increased (P<0.05), the number of Aβ and the value of IOD were decreased in CA1 region and DG region of hippocampus (P<0.05), the protein expression of HMGB1, RAGE, TLR4, NF-κB p65 was significantly increased (P<0.05). Conclusion: Oral curcumin for 5 months reverses the learning and memory impairment of APP/PS1 transgenic mice, and the mechanism is likely to inhibit HMGB1-RAGE/TLR4-NF-κB signaling pathway.

Key words： curcumin APP/PS1 double transgenic mice high mobility group box protein-1 receptor for advanced glycation endproducts Toll-like receptor-4 nuclear factor kappa B

Received: 10 September 2017

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	HAN Yuan
	HUANG Chenmiao
	FANG Qianjuan
	LIU Qixing
	NAN Ke
	XIANG Fangfang
	CAO Hong
	LI Jun

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I3/157

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