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| Hirsutine mitigates high-fat-induced apoptosis in H9C2 cells by activating mitophagy |
| SHENG Sunpeng1,CHEN Shijia2, DONG Xicheng3, DING Lu4, MA Jianshe4, JIN Peifeng1. |
| 1.Department of Cardiac Surgery,the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, China; 2.The First School of Medicine, School of Information and Engineering, Wenzhou Medical University, Wenzhou 325035, China;3.School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou 325035, China; 4.School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325035, China |
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| Cite this article: |
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SHENG Sunpeng,CHEN Shijia,DONG Xicheng, et al. Hirsutine mitigates high-fat-induced apoptosis in H9C2 cells by activating mitophagy[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2024, 54(10): 791-797,805.
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Abstract Objective: To explore the effect of Hirsutine on mitophagy and apoptosis in H9C2 cells induced by high-fat. Methods: With untreated H9C2 cells served as the control group (Control), H9C2 cells stimulated by palmitic acid were used as the high-fat group (PA), H9C2 cells treated with Hirsutine as the treatment group (PA+HS) and H9C2 cells treated with the Rho kinase inhibitor Y-27632 as the inhibitor group (PA+HS+Y-27632).CCK8 assay was used to evaluate cell viability; TUNEL staining was used to detect cell apoptosis; TMRE staining was used to detect changes in mitochondrial membrane potential (mPTP); Western blot was used to detect the expression of mitophagy proteins LC3I/II, Pink 1 and Parkin as well as apoptosis-related proteins Cleavedcaspase 3, caspase 3, Bax, and Bcl-2. Results: Compared with the control group, the viability of cells treated with 200, 400, and 800 μmol/L PA decreased (P<0.05); the TMRE fluorescence intensity was significantly weaker,while the expression of LC3-I/II, Pink , and Parkin was significantly lower (P<0.05); the TUNEL-positive cells increased significantly, and the expression of Cleaved-caspase 3/caspase 3 and Bax/Bcl-2 increased significantly (P<0.05) in the PA group. Compared with the PA group, the TMRE fluorescence intensity was significantly stronger; the expression of LC3-I/II, Pink , and Parkin was significantly higher (P<0.05); the TUNEL-positive cells decreased significantly, and the expression of Cleaved-caspase 3/caspase 3 and Bax/Bcl-2 decreased significantly (P<0.05) in the PA+HS group. Compared with the PA+HS group, the TMRE fluorescence intensity was weaker; the expression of LC3-I/II, Pink , and Parkin was significantly lower (P<0.05); the TUNELpositive cells increased significantly, and the expression of Cleaved-caspase 3/caspase 3 and Bax/Bcl-2 increased significantly (P<0.05) in the PA+HS+Y-27632 group. Conclusion: Hirsutine alleviates apoptosis of H9C2 cells induced by high-fat through the activation of mitophagy.
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Received: 15 January 2024
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