ZAG对结肠腺癌癌症恶病质小鼠脂质代谢的影响

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摘要目的：通过小鼠结肠腺癌癌症恶病质（CC）模型探索ZAG对CC机体脂质代谢的影响。方法：BALB/c小鼠皮下接种小鼠结肠癌colon-26细胞，构建结肠腺癌模型，BALB/c小鼠根据造模情况分为健康对照组（HC组）、肿瘤组（TC组）和肿瘤恶病质组（CC组），每组6只。取TC组和CC组的癌旁组织和肿瘤组织检测ZAG mRNA和蛋白表达水平；3组小鼠皮下脂肪组织检测ZAG蛋白浓度和mRNA表达水平，并分析TC组和CC组ZAG mRNA表达水平与体质量减轻的相关性；取3组小鼠血清，检测ZAG和脂联素的mRNA水平，并分析TC组和CC组两者的相关性。构建小鼠结肠腺癌CC模型，分别注射pcDNA、pcDNA-ZAG，观察并记录小鼠体质量变化情况。结果：CC组肿瘤组织和脂肪组织中ZAG mRNA和蛋白水平显著高于TC组（P＜0.05），且脂肪组织中ZAG mRNA水平与体质量减轻值之间呈正相关；CC组血清中ZAG和脂联素的mRNA水平显著高于TC组（P＜0.05），且两者呈正相关；注射pcDNA-ZAG的结肠腺癌CC小鼠体质量明显降低（P＜0.05）。结论：ZAG能显著促进脂肪分解，影响结肠腺癌CC小鼠体质量。

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关键词 ：结肠腺癌, 癌症恶病质, ZAG, 脂联素, 体质量减轻, 小鼠

Abstract：Objective: To investigate the effect of zinc-alpha2-glycoprotein (ZAG) on lipid metabolism of mice with cancer cachexia (CC) using a mouse model of colon adenocarcinoma cachexia. Methods: A mouse model of colon adenocarcinoma cachexia was well established by inoculating mouse colon cancer cell line, colon 26 into BALB/c mice. A total of 18 BALB/c mice were evenly divided into 3 groups randomly: healthy control (HC) group, tumor control (TC) group and cancer cachexia (CC) group (n=6). Adjacent non-tumor tissues and adenocarcinoma of colon (AC) tissues isolated from TC group and CC group were collected to determine ZAG mRNA and protein level. ZAG protein concentration and mRNA level in subcutaneous adipose tissues of each group were determined, while the correlation between ZAG mRNA level and weight loss was confirmed. mRNA expression level of ZAG and adiponectin in serum were measured, and the correlation between them in TC group and CC group was analyzed. Finally, weight was recorded in cancer cachexitic model of mice with colon adenocarcinoma transplantation followed by adenovirus-constructed pcDNA or pcDNA-ZAG injection. Results: ZAG mRNA and protein level of AC tissues and adipose tissues in CC group was significantly higher than those in TC group (P<0.05). ZAG mRNA level was correlated positively with weight loss in adipose tissue. Besides, the serum mRNA levels of ZAG and adiponectin in CC group were significantly higher than those in TC group and were positively correlated. In addition, mice weight was observably reduced after treatment with pcDNA-ZAG (P<0.05). Conclusion: Enhanced ZAG can significantly promote the steatolysis, and affect the weight in colon adenocarcinoma cachexia mice.

Key words： colon adenocarcinoma cancer cachexia zinc-alpha2-glycoprotein adiponectin weight loss mice

收稿日期: 2017-05-27

基金资助:温州市科技计划项目(Y20120015)。

通讯作者: 余震，主任医师，教授，Email：yuzhen0577@gmail.com。

作者简介: 张培趁（1977-），男，浙江苍南人，主治医师。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2018/V48/I2/101

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