长链非编码RNA CasC7对宫颈癌细胞增殖的影响

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摘要目的：探讨长链非编码RNA（lncRNA）CasC7在宫颈癌中的表达水平以及对宫颈癌发生发展的影响。方法：利用生物信息学方法分析宫颈癌芯片GSE9750中lncRNA CasC7的表达情况。采用实时荧光定量PCR（qRT-PCR）法检测宫颈癌组织、宫颈上皮内瘤变（CIN）组织、正常宫颈组织及正常宫颈上皮细胞（H8细胞）、宫颈癌细胞（SiHa、CaSki和HeLa细胞）中lncRNA CasC7的表达量，并分析CasC7的表达水平与宫颈癌临床病理特征的关系。同时，采用CCK-8实验检测下调CasC7的表达对宫颈癌细胞增殖情况的影响。结果：lncRNA CasC7在宫颈癌组织以及CIN组织中的表达水平较正常宫颈组织中高（P＜0.01）。与H8细胞相比，CasC7在SiHa、CaSki和HeLa细胞中的表达量均较高（P＜0.05）。临床数据分析显示，CasC7的表达水平与FIGO分期（P=0.001）、肿瘤分化程度相关（P=0.015），而与年龄、肿瘤大小等因素无关（P＞0.05）。CCK-8实验结果表明下调CasC7的表达能抑制SiHa、CaSki和HeLa细胞的增殖（P＜0.05）。结论：lncRNA CasC7在宫颈癌的发生发展过程中可能是起促癌基因的作用。

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	段萍

关键词 ：长链非编码RNA, CasC7, 宫颈肿瘤, 细胞增殖

Abstract：Objective: To investigate the expression level of lncRNA CasC7 in cervical cancer and its role in tumorigenesis and development of cervical cancer. Methods: Bioinformatics was utilized to analyse the expression level of lncRNA CasC7 in GSE9750, a gene expression microarrays for cervical cancer. Quantitative real-time PCR (qRT-PCR) was used to detect the expression level of CasC7 in cervical cancer tissues, cervical intraepithelial neoplasia tissues, normal cervical tissues and normal cervical epithelial cells (H8 cells), cervical cancer cells (SiHa cells, CaSki cells and HeLa cells). Association between the expression of CasC7 and clinicopathological features of cervical cancer was analyzed. Meanwhile, the CCK-8 assays was used to measured cell proliferation when CasC7 expression was inhibited. Results: LncRNA expression in cervical cancer and cervical intraepithelial neoplasia was higher than that in normal cervical tissues (P<0.01). Compared with normal cervical epithelial cells, CasC7 was up-regulated in SiHa cells, CaSki cells and HeLa cells (P<0.05). High expression of CasC7 was correlated with advanced FIGO stage (P=0.001) and poor differentiation (P=0.015). However, neither patients age nor tumor size was associated with the expression of CasC7 (P>0.05). CCK-8 assays showed that cell proliferation of SiHa cells, CaSki cells and HeLa cells was suppressed by CasC7 knockdown (P<0.05). Conclusion: CasC7 may act as an oncogene during the development of cervical cancer.

Key words： long noncoding RNA CasC7 uterine cervical neoplasms cell proliferation

收稿日期: 2017-03-11

基金资助:浙江省自然科学基金资助项目（Y16H160214）。

通讯作者: 段萍，教授，硕士生导师，Email：dppddpp@126.com。

作者简介: 严德文（1979-），男，浙江台州人，副主任医师。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2018/V48/I2/91

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